Crystallization and preliminary X‐ray analysis of monalysin, a novel β‐pore‐forming toxin from the entomopathogen Pseudomonas entomophila. Issue 8 (31st July 2013)

• Record Type: Journal Article
• Authors: Blemont, Maryline; Vincentelli, Renaud; Kellenberger, Christine; Opota, Onya; Lemaitre, Bruno; Roussel, Alain; Leone, Philippe
• Is Part Of: Acta crystallographica. Volume 69:Issue 8(2013:Aug.)
• Page Start: 930
• Page End: 933
• Publication Date: 2013-07-31
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S174430911301885X
• Languages: English
• ISSNs: 1744-3091

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Monalysin was recently described as a novel pore‐forming toxin (PFT) secreted by the <italic>Drosophila</italic> pathogen <italic>Pseudomonas entomophila</italic>. Recombinant monalysin is multimeric in solution, whereas PFTs are supposed to be monomeric until target membrane association. Monalysin crystals were obtained by the hanging‐drop vapour‐diffusion method using PEG 8000 as precipitant. Preliminary X‐ray diffraction analysis revealed that monalysin crystals belonged to the monoclinic space group <italic>C</italic>2, with unit‐cell parameters <italic>a</italic> = 162.4, <italic>b</italic> = 146.2, <italic>c</italic> = 144.4 Å, β = 122.8°, and diffracted to 2.85 Å resolution using synchrotron radiation. Patterson self‐rotation analysis and Matthews coefficient calculation indicate that the asymmetric unit contains nine copies of monalysin. Heavy‐atom derivative data were collected and a Ta₆Br₁₄ cluster derivative data set confirmed the presence of ninefold noncrystallographic symmetry.</p> </abstract>