Crystallization and preliminary X‐ray crystallographic analysis of UDP‐glucuronic acid:flavonol‐3‐O‐glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera. Issue 1 (8th January 2013)

• Record Type: Journal Article
• Authors: Mizohata, Eiichi; Okuda, Takuma; Hatanaka, Seika; Nakayama, Taisuke; Horikawa, Manabu; Nakayama, Toru; Ono, Eiichiro; Inoue, Tsuyoshi
• Is Part Of: Acta crystallographica. Volume 69:Issue 1(2013:Jan.)
• Page Start: 65
• Page End: 68
• Publication Date: 2013-01-08
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S1744309112045095
• Languages: English
• ISSNs: 1744-3091

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Grapevine (<italic>Vitis vinifera</italic>) glycosyltransferase 5 (VvGT5) is a UDP‐glucuronic acid:flavonol‐3‐<italic>O</italic>‐glucuronosyltransferase that catalyses the 3‐<italic>O</italic>‐specific glucuronosylation of flavonols using UDP‐glucuronic acid as a sugar donor to produce flavonol 3‐<italic>O</italic>‐glucosides, which are important bioactive phytochemicals. Recombinant VvGT5 expressed in <italic>Escherichia coli</italic> cells was purified and crystallized by the sitting‐drop vapour‐diffusion method. A full set of X‐ray diffraction data was collected to 2.2 Å Bragg spacing from a single crystal using a synchrotron‐radiation source. The crystal was hexagonal, belonging to space group <italic>P</italic>6₁22, with unit‐cell parameters <italic>a</italic> = <italic>b</italic> = 102.70, <italic>c</italic> = 535.92 Å. The initial phases were determined by the molecular‐replacement method.</p> </abstract>