Expression, purification, crystallization and preliminary X‐ray crystallographic study of the nucleocapsid protein of Tomato spotted wilt virus. Issue 6 (31st May 2013)

• Record Type: Journal Article
• Authors: Komoda, Keisuke; Narita, Masanori; Tanaka, Isao; Yao, Min
• Is Part Of: Acta crystallographica. Volume 69:Issue 6(2013:Jun.)
• Page Start: 700
• Page End: 703
• Publication Date: 2013-05-31
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S174430911301302X
• Languages: English
• ISSNs: 1744-3091

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Tomato spotted wilt virus</italic> (TSWV), which causes severe damage to various agricultural crops such as tomato, pepper, lettuce and peanut, is a negative‐stranded RNA virus belonging to the <italic>Tospovirus</italic> genus of the <italic>Bunyaviridae</italic> family. Viral genomic RNA molecules are packaged in the form of ribonucleoprotein complexes, each of which contains one viral RNA molecule that is coated with many nucleocapsid (N) proteins. Here, the expression and crystallization of TSWV N protein are reported. Native and selenomethionine‐substituted crystals of N protein belonged to the same space group <italic>P</italic>2₁. Their unit‐cell parameters were <italic>a</italic> = 66.8, <italic>b</italic> = 97.2, <italic>c</italic> = 72.0 Å, β = 112.8° and <italic>a</italic> = 66.5, <italic>b</italic> = 96.3, <italic>c</italic> = 72.1 Å, β = 113.4°, respectively.</p> </abstract>