Crystallization and preliminary X‐ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1. Issue 10 (8th October 2013)

• Record Type: Journal Article
• Authors: Wan, Li; Williams, Simon J.; Zhang, Xiaoxiao; Ericsson, Daniel J.; Koeck, Markus; Dodds, Peter N.; Ellis, Jeffrey G.; Kobe, Bostjan
• Is Part Of: Acta crystallographica. Volume 69:Issue 10(2013:Oct.)
• Page Start: 1094
• Page End: 1096
• Publication Date: 2013-10-08
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S1744309113023142
• Languages: English
• ISSNs: 1744-3091

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The plant hormones cytokinins play a central role in regulating cell division and developmental events. Cytokinin oxidase regulates the levels of these plant hormones by catalyzing their irreversible oxidation, which contributes to the regulation of various morpho‐physiological processes controlled by cytokinins. In this study, the crystallization and preliminary X‐ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1 are reported. Plate‐like crystals of LuCKX1.1 were obtained using PEG 3350 as a precipitant and diffracted X‐rays to 1.78 Å resolution. The protein crystals have the symmetry of space group <italic>C</italic>2 and are most likely to contain two molecules per asymmetric unit.</p> </abstract>