A quantitative approach to histopathological dissection of elastin‐related disorders using multiphoton microscopy. (10th October 2013)

• Record Type: Journal Article
• Authors: Tong, P.L.; Qin, J.; Cooper, C.L.; Lowe, P.M.; Murrell, D.F.; Kossard, S.; Ng, L.G.; Roediger, B.; Weninger, W.; Haass, N.K.
• Is Part Of: British journal of dermatology. Volume 169:Number 4(2013:Oct.)
• Page Start: 869
• Page End: 879
• Publication Date: 2013-10-10
• Subjects: Dermatology -- Periodicals; Skin -- Diseases -- Periodicals; 616.5
• DOI: 10.1111/bjd.12430
• Languages: English
• ISSNs: 0007-0963

Abstract

<abstract abstract-type="short" xml:lang="en" id="bjd12430-abs-0002"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>What's already known about this topic?</bold> </p> <p> <list id="bjd12430-list-0001" list-type="bullet"> <list-item> <p>Multiphoton microscopy has been used on tissue sections and in patients to assess skin pathologies.</p> </list-item> <list-item> <p>Autofluorescent structures within the skin, including elastic fibres, can be visualized using multiphoton imaging without the need for histochemical or antibody staining.</p> </list-item> </list> </p> <p> <bold>What does this study add?</bold> </p> <p> <list id="bjd12430-list-0002" list-type="bullet"> <list-item> <p>Development of the Dermal Elastin Morphology Index (DEMI) to enable objective three‐dimensional quantification of elastic fibres.</p> </list-item> <list-item> <p>Use of DEMI is demonstrated in elastin‐related skin disorders.</p> </list-item> <list-item> <p>DEMI can be used on tissue sections, where it correlates with histopathology, but in contrast to histopathology it allows <italic>in vivo</italic> application.</p> </list-item> </list> </p> </abstract>