Three‐dimensional structure of RBcel1, a metagenome‐derived psychrotolerant family GH5 endoglucanase. Issue 8 (31st July 2013)

• Record Type: Journal Article
• Authors: Delsaute, Maud; Berlemont, Renaud; Dehareng, Dominique; Van Elder, Dany; Galleni, Moreno; Bauvois, Cédric
• Is Part Of: Acta crystallographica. Volume 69:Issue 8(2013:Aug.)
• Page Start: 828
• Page End: 833
• Publication Date: 2013-07-31
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S1744309113014565
• Languages: English
• ISSNs: 1744-3091

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>RBcel1 is an endoglucanase belonging to glycoside hydrolase family 5 subfamily 5 (GH5_5) that was recently identified from a soil metagenome library from the Antarctic. Unlike its closest structural homologue (Cel5A from <italic>Thermoascus aurantiacus</italic>), this enzyme was reported to be able to catalyze transglycosylation reactions and has putatively been implicated in the bacterial cellulose‐synthesis process. Here, the structure of RBcel1 at 1.4 Å resolution, solved by molecular replacement, is reported. The structure and putative substrate‐binding site are described and compared with those of other GH5_5 subfamily members.</p> </abstract>