Cloning, expression, purification and preliminary X‐ray diffraction studies of a mycobacterial protein implicated in bacterial survival in macrophages. Issue 5 (22nd May 2013)

• Record Type: Journal Article
• Authors: Shahine, Adam E.; Chan, Phooi Y.; Littler, Dene; Vivian, Julian; Brammananth, Rajini; Crellin, Paul K.; Coppel, Ross L.; Rossjohn, Jamie; Beddoe, Travis
• Is Part Of: Acta crystallographica. Volume 69:Issue 5(2013:May)
• Page Start: 566
• Page End: 569
• Publication Date: 2013-05-22
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S1744309113010427
• Languages: English
• ISSNs: 1744-3091

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Mycobacterium</italic> species have developed numerous strategies to avoid the antimycobacterial actions of macrophages, enabling them to survive within the generally inhospitable environment of the cell. The recently identified MSMEG_5817 protein from <italic>M. smegmatis</italic> is highly conserved in <italic>Mycobacterium</italic> spp. and is required for bacterial survival in macrophages. Here, the cloning, expression, purification and crystallization of MSMEG_5817 is reported. Crystals of MSMEG_5817 were grown in 1.42 <italic>M</italic> Li₂SO₄, 0.1 <italic>M</italic> Tris–HCl pH 7.7, 0.1 <italic>M</italic> sodium citrate tribasic dihydrate. Native and multiple‐wavelength anomalous dispersion (MAD) data sets have been collected and structure determination is in progress.</p> </abstract>