Crystallization and preliminary crystallographic study of oligomers of the haemolytic lectin CEL‐III from the sea cucumber Cucumaria echinata. Issue 4 (2nd April 2013)

• Record Type: Journal Article
• Authors: Unno, Hideaki; Hisamatsu, Keigo; Nagao, Tomonao; Tateya, Yuki; Matsumoto, Naoki; Goda, Shuichiro; Hatakeyama, Tomomitsu
• Is Part Of: Acta crystallographica. Volume 69:Issue 4(2013:Apr.)
• Page Start: 416
• Page End: 420
• Publication Date: 2013-04-02
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S1744309113004065
• Languages: English
• ISSNs: 1744-3091

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>CEL‐III is a Ca²⁺‐dependent haemolytic lectin isolated from the marine invertebrate <italic>Cucumaria echinata</italic>. This lectin binds to Gal/GalNAc‐containing carbohydrate chains on the cell surface and, after conformational changes, oligomerizes to form ion‐permeable pores in cell membranes. CEL‐III also forms soluble oligomers similar to those formed in cell membranes upon binding of specific carbohydrates in high‐pH and high‐salt solutions. These soluble and membrane CEL‐III oligomers were crystallized and X‐ray diffraction data were collected. Crystals of soluble oligomers and membrane oligomers diffracted X‐rays to 3.3 and 4.2 Å resolution, respectively, using synchrotron radiation and the former was found to belong to space group <italic>C</italic>2. Self‐rotation functional analysis of the soluble oligomer crystal suggested that it might be composed of heptameric CEL‐III.</p> </abstract>