Crystallization and preliminary X‐ray diffraction analysis of the Nif3‐family protein MJ0927 from Methanocaldococcus jannaschii. Issue 1 (8th January 2013)

• Record Type: Journal Article
• Authors: Kuan, Shu‐Min; Chen, Hsiao‐Chi; Huang, Chi‐Hung; Chang, Chi‐Huang; Chen, Sheng‐Chia; Yang, Chia Shin; Chen, Yeh
• Is Part Of: Acta crystallographica. Volume 69:Issue 1(2013:Jan.)
• Page Start: 80
• Page End: 82
• Publication Date: 2013-01-08
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S1744309112049408
• Languages: English
• ISSNs: 1744-3091

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>MJ0927 is a member of the Nif3 family and is widely distributed across living organisms. Although several crystal structures of Nif3 proteins have been reported, structural information on archaeal Nif3 is still limited. To understand the structural differences between bacterial and archaeal Nif3 proteins, MJ0927 from <italic>Methanocaldococcus jannaschii</italic> was purified and crystallized using the sitting‐drop vapour‐diffusion method. The crystals diffracted to a resolution of 2.47 Å and belonged to the orthorhombic space group <italic>C</italic>222, with unit‐cell parameters <italic>a</italic> = 81.21, <italic>b</italic> = 172.94, <italic>c</italic> = 147.42 Å. Determination of this structure may provide insights into the function of MJ0927.</p> </abstract>