Distribution of edin in Staphylococcus aureus isolated from diabetic foot ulcers. (23rd November 2012)

• Record Type: Journal Article
• Authors: Messad, N.; Landraud, L.; Canivet, B.; Lina, G.; Richard, J.‐L.; Sotto, A.; Lavigne, J.‐P.; Lemichez, E.; Raoult, D.
• Is Part Of: Clinical microbiology and infection. Volume 19:Number 9(2013:Sep.)
• Page Start: 875
• Page End: 880
• Publication Date: 2012-11-23
• Subjects: Medical microbiology -- Periodicals; Diagnostic microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.01
• DOI: 10.1111/1469-0691.12084
• Languages: English
• ISSNs: 1198-743X

Abstract

<abstract abstract-type="main" id="clm12084-abs-0001"> <title>Abstract</title> <p> <italic>Staphylococcus aureus</italic> is both a common colonizer of human skin and the most frequently isolated pathogen in diabetes foot infections (DFIs). The spread of DFI to soft tissue and bony structures is a major causal factor for lower‐limb amputation. It is therefore of great importance to differentiate colonizing from infecting strains of <italic>S. aureus</italic>. Epidermal cell differentiation inhibitors known as EDIN and EDIN‐like factors, a group of toxins targeting RhoA master regulator of the actin cytoskeleton, may confer virulence properties on <italic>S. aureus</italic>. In this study, for the first time, analysis of <italic>S. aureus</italic> strains, recovered in DFIs at an initial stage and during the follow‐up, showed that 71.4% of <italic>edin</italic>‐positive strains were associated with moderate‐to‐severe infections (grades 3 and 4 of the IDSA/IWGDF classification) compared with 28.6% of <italic>edin</italic>‐positive strains associated with low‐grade infections. Most of these strains were <italic>edin‐B</italic> positive (86.7%) and belonged to CC25/28‐MSSA (<italic>n</italic> = 10). One <italic>edin‐B</italic>‐positive ST152‐MSSA strain was negative for the two highly prevalent predictive markers of infecting strains (<italic>lukDE</italic> and <italic>hlgv</italic>). Collectively, this points towards the <italic>edin‐B</italic> encoding gene as a <italic>bonafide</italic> subsidiary predictive risk marker of DFI.</p> </abstract>