Hypoxia induces re‐entry of committed cells into pluripotency. (4th October 2013)

• Record Type: Journal Article
• Authors: Mathieu, Julie; Zhang, Zhan; Nelson, Angelique; Lamba, Deepak A.; Reh, Thomas A.; Ware, Carol; Ruohola‐Baker, Hannele
• Is Part Of: Stem cells. Volume 31:Number 9(2013:Sep.)
• Page Start: 1737
• Page End: 1748
• Publication Date: 2013-10-04
• Subjects: Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.1446
• Languages: English
• ISSNs: 1066-5099

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>Adult stem cells reside in hypoxic niches, and embryonic stem cells (ESCs) are derived from a low oxygen environment. However, it is not clear whether hypoxia is critical for stem cell fate since for example human ESCs (hESCs) are able to self‐renew in atmospheric oxygen concentrations as well. We now show that hypoxia can govern cell fate decisions since hypoxia alone can revert hESC‐ or iPSC‐derived differentiated cells back to a stem cell‐like state, as evidenced by re‐activation of an Oct4‐promoter reporter. Hypoxia‐induced "de‐differentiated" cells also mimic hESCs in their morphology, long‐term self‐renewal capacity, genome‐wide mRNA and miRNA profiles, Oct4 promoter methylation state, cell surface markers TRA1–60 and SSEA4 expression, and capacity to form teratomas. These data demonstrate that hypoxia can influence cell fate decisions and could elucidate hypoxic niche function. S<sc>tem</sc> C<sc>ells</sc><italic>2013;31:1737‐1748</italic></p> </abstract>