Ex vivo antimicrobial efficacy of strong acid electrolytic water against Enterococcus faecalis biofilm. (13th March 2013)

• Record Type: Journal Article
• Authors: Chen, X.; Li, P.; Wang, X.; Gu, M.; Zhao, C.; Sloan, A. J.; Lv, H.; Yu, Q.
• Is Part Of: International endontic journal. Volume 46:Number 10(2013:Oct.)
• Page Start: 938
• Page End: 946
• Publication Date: 2013-03-13
• Subjects: Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12084
• Languages: English
• ISSNs: 0143-2885

Abstract

<abstract abstract-type="main" xml:lang="en" id="iej12084-abs-0001"> <title>Abstract</title> <sec id="iej12084-sec-0001" sec-type="section"> <title>Aim</title> <p>To observe the antimicrobial effect of strong acid electrolytic water (SAEW) against an <italic>Enterococcus faecalis</italic> (<italic>E. faecalis</italic>) biofilm when used as a root canal irrigant.</p> </sec> <sec id="iej12084-sec-0002" sec-type="section"> <title>Methodology</title> <p>The effect of SAEW, sodium hypochlorite (5.25%; NaOCl) and sodium chloride (0.9%; normal saline) on <italic>E. faecalis</italic> biofilm vitality on coverslips was observed by confocal laser scanning microscopy (CLSM). Thirty‐five root canals from extracted human teeth were sterilized prior to contamination with <italic>E. faecalis</italic> for four weeks. Bacterial samples were collected with sterile paper points and plated onto BHI agar plates for 48 h. Root canal walls were observed by scanning electron microscopy before and after instrumentation, together with root canal irrigation with SAEW, NaOCl or normal saline, with or without ultrasonic vibration. Antimicrobial effectiveness was established by counting colony‐forming units and analysed by two‐way <sc>anova</sc>.</p> </sec> <sec id="iej12084-sec-0003" sec-type="section"> <title>Results</title> <p>Confocal laser scanning microscopy revealed that SAEW decreased <italic>E. faecalis</italic> biofilm vitality, and the proportion of dead bacteria increased in accordance with increasing treatment time. Most bacteria in the biofilms were killed after 10‐min treatment. No significant difference was observed between SAEW and NaOCl groups at the same treatment time (<italic>P </italic>><italic> </italic>0.05) or in the susceptibility of <italic>E. faecalis</italic> to SAEW and NaOCl (<italic>P </italic>><italic> </italic>0.05) in extracted human teeth with or without ultrasonic activation. SAEW and NaOCl were more effective against <italic>E. faecalis</italic> biofilm than normal saline, and antimicrobial efficacy was significantly enhanced by ultrasonic vibration (<italic>P </italic><<italic> </italic>0.05).</p> </sec> <sec id="iej12084-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Strong acid electrolytic water effectively killed <italic>E. faecalis</italic> in a biofilm both on coverslips and in the root canals of extracted human teeth.</p> </sec> </abstract>