Ultratight crystal packing of a 10 kDa protein. (24th March 2013)

• Record Type: Journal Article
• Authors: Trillo‐Muyo, Sergio; Jasilionis, Andrius; Domagalski, Marcin J.; Chruszcz, Maksymilian; Minor, Wladek; Kuisiene, Nomeda; Arolas, Joan L.; Solà, Maria; Gomis‐Rüth, F. Xavier
• Is Part Of: Acta crystallographica. Volume 69:Part 3(2013:Mar.)
• Page Start: 464
• Page End: 470
• Publication Date: 2013-03-24
• Subjects: Biomolecules -- Structure -- Periodicals; Physical biochemistry -- Periodicals; X-ray crystallography -- Periodicals; Crystallography -- Periodicals; 572
• DOI: 10.1107/S0907444912050135
• Languages: English
• ISSNs: 0907-4449

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>While small organic molecules generally crystallize forming tightly packed lattices with little solvent content, proteins form air‐sensitive high‐solvent‐content crystals. Here, the crystallization and full structure analysis of a novel recombinant 10 kDa protein corresponding to the C‐terminal domain of a putative U32 peptidase are reported. The orthorhombic crystal contained only 24.5% solvent and is therefore among the most tightly packed protein lattices ever reported.</p> </abstract>