A comparative analysis of total serum miRNA profiles identifies novel signature that is highly indicative of metastatic melanoma: a pilot study. (September 2013)

• Record Type: Journal Article
• Authors: Greenberg, Eyal; Besser, Michal J.; Ben-Ami, Eytan; Shapira-Frommer, Ronnie; Itzhaki, Orit; Zikich, Dragoslav; Levy, Daphna; Kubi, Adva; Eyal, Eran; Onn, Amir; Cohen, Yehudit; Barshack, Iris; Schachter, Jacob; Markel, Gal
• Is Part Of: Biomarkers. Volume 18:Number 6(2013)
• Page Start: 502
• Page End: 508
• Publication Date: 2013-09
• Subjects: Biochemical markers -- Periodicals; 610.28
• DOI: 10.3109/1354750X.2013.816777
• Languages: English
• ISSNs: 1354-750X

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Quantification of circulating microRNAs (miRNAs) has recently become feasible and reliable, with most efforts focusing on miRNAs overexpressed by cancer cells.</p> <p> <italic>Objective</italic>: Identification of a characteristic circulating miRNAs profile in melanoma patients.</p> <p> <italic>Methods</italic>: We conducted a pilot study comprised of unbiased qPCR comparison of serum miRNA profiles between metastatic melanoma patients and healthy donors.</p> <p> <italic>Results</italic>: Loss of two normal serum-miRNAs, miR-29c and miR-324-3p, is highly indicative of metastatic melanoma. Hierarchical clustering analysis supported the results and clearly distinguished melanoma patients from healthy donors, metastatic colon and renal cancer patients.</p> <p> <italic>Discussion and conclusions</italic>: This approach is independent of tumor heterogeneity and is expected to have superior biomarker performances.</p> </abstract>