Changes in aberrant DNA methylation after Helicobacter pylori eradication: A long‐term follow‐up study. Issue 9 (12th June 2013)

• Record Type: Journal Article
• Authors: Shin, Cheol Min; Kim, Nayoung; Lee, Hye Seung; Park, Ji Hyun; Ahn, Soyeon; Kang, Gyeong Hoon; Kim, Jung Mogg; Kim, Joo Sung; Lee, Dong Ho; Jung, Hyun Chae
• Is Part Of: International journal of cancer. Volume 133:Issue 9(2013:Nov. 01)
• Page Start: 2034
• Page End: 2042
• Publication Date: 2013-06-12
• Subjects: Cancer -- Periodicals; Cancer -- Prevention -- Periodicals; 616.994
• DOI: 10.1002/ijc.28219
• Languages: English
• ISSNs: 0020-7136

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Changes of DNA methylation in gastric mucosae after eradication of <italic>Helicobacter pylori</italic> have not been clarified yet. From this background, we investigated time course of DNA methylation following <italic>H. pylori</italic> eradication in 221 successfully <italic>H. pylori</italic> eradicated subjects with endoscopic follow‐up at least for 6 months, including 114 controls, 53 subjects with gastric dysplasia and 54 patients with early gastric cancer. All dysplasia and gastric cancer patients underwent endoscopic resection at the time of enrollment. The methylation levels in <italic>LOX</italic>, <italic>APC</italic> and <italic>MOS</italic> genes from noncancerous gastric mucosae using quantitative methylation‐specific PCR, as well as the histologic findings of gastric mucosae, were compared before and after eradication. Average follow‐up duration was 26.0 months (range: 6 to 76 months). <italic>H. pylori</italic> eradication decreased methylation levels in <italic>LOX</italic> (<italic>p‐</italic>value for slope < 0.001) but not in <italic>APC</italic>. In <italic>MOS</italic>, decrease of its methylation level following <italic>H. pylori</italic> eradication was significant among controls without intestinal metaplasia (IM) (<italic>p‐</italic>value for slope < 0.05); however, it was not observed among patients with IM or those with dysplasia or gastric cancer. After <italic>H. pylori</italic> eradication, methylation level in <italic>MOS</italic> persistently increased in patients with dysplasia or gastric cancer (<italic>p</italic> < 0.01). In conclusion, <italic>H. pylori</italic> eradication decreases aberrant DNA methylation with gene‐specific manner. Methylation level in <italic>MOS</italic> is associated with IM and may be used as a surrogate marker for gastric cancer risk, regardless of <italic>H. pylori</italic> eradication history.</p> </abstract>