Investigating glucose and xylose metabolism in Saccharomyces cerevisiae and Scheffersomyces stipitis via 13C metabolic flux analysis. Issue 9 (1st August 2013)

• Record Type: Journal Article
• Authors: Feng, Xueyang; Zhao, Huimin
• Is Part Of: AIChE journal. Volume 59:Issue 9(2013:Sep.)
• Page Start: 3195
• Page End: 3202
• Publication Date: 2013-08-01
• Subjects: Chemical engineering -- Periodicals; Génie chimique -- Périodiques; 660.28
• DOI: 10.1002/aic.14182
• Languages: English
• ISSNs: 0001-1541

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> ¹³C metabolic flux analysis (¹³CMFA) has been extensively applied in studying the glucose metabolism of yeast strains such as <italic>Saccharomyces cerevisiae</italic> and <italic>Scheffersomyces stipitis</italic>. Here, we tried to augment the previous fluxomic studies by applying ¹³CMFA to rigorously investigate the metabolic flux distributions in <italic>S. stipitis</italic> and the recombinant <italic>S. cerevisiae</italic> strains when xylose was utilized as the sole carbon substrate. It was found that less carbon fluxes were diverted into the TCA cycle in <italic>S. stipitis</italic> than the recombinant <italic>S. cerevisiae</italic> strains. Compared to single sugar utilization, the co‐utilization of glucose and xylose by <italic>S. stipitis</italic> led to increased metabolic fluxes into the futile pathway and the TCA cycle, but did not improve sugar‐based ethanol yield. In addition, heterologous expression of xylose pathway in engineered <italic>S. cerevisiae</italic> strains may affect the glucose utilization. © 2013 American Institute of Chemical Engineers <italic>AIChE J</italic>, 59: 3195–3202, 2013</p> </abstract>