Increased T Cell Glucose Uptake Reflects Acute Rejection in Lung Grafts. Issue 10 (8th August 2013)

• Record Type: Journal Article
• Authors: Chen, D. L.; Wang, X.; Yamamoto, S.; Carpenter, D.; Engle, J. T.; Li, W.; Lin, X.; Kreisel, D.; Krupnick, A. S.; Huang, H. J.; Gelman, A. E.
• Is Part Of: American journal of transplantation. Volume 13:Issue 10(2013)
• Page Start: 2540
• Page End: 2549
• Publication Date: 2013-08-08
• Subjects: Transplantation of organs, tissues, etc -- Periodicals; 617.95
• DOI: 10.1111/ajt.12389
• Languages: English
• ISSNs: 1600-6135

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="ajt12389-sec-0001" sec-type="section"> <p>Although T cells are required for acute lung rejection, other graft–infiltrating cells such as neutrophils accumulate in allografts and are also high glucose utilizers. Positron emission tomography (PET) with the glucose probe [¹⁸F]fluorodeoxyglucose ([¹⁸F]FDG) has been employed to image solid organ acute rejection, but the sources of glucose utilization remain undefined. Using a mouse model of orthotopic lung transplantation, we analyzed glucose probe uptake in the grafts of syngeneic and allogeneic recipients with or without immunosuppression treatment. Pulmonary microPET scans demonstrated significantly higher [¹⁸F]FDG uptake in rejecting allografts when compared to transplanted lungs of either immunosuppressed or syngeneic recipients. [¹⁸F]FDG uptake was also markedly attenuated following T cell depletion therapy in lung recipients with ongoing acute rejection. Flow cytometric analysis using the fluorescent deoxyglucose analog 2‐NBDG revealed that T cells, and in particular CD8⁺ T cells, were the largest glucose utilizers in acutely rejecting lung grafts followed by neutrophils and antigen‐presenting cells. These data indicate that imaging modalities tailored toward assessing T cell metabolism may be useful in identifying acute rejection in lung recipients.</p> </sec> </abstract>