Anthocyanin Phytochemical Profiles and Anti‐oxidant Activities of Vitis candicans and Vitis doaniana. Issue 5 (9th July 2013)

• Record Type: Journal Article
• Authors: la, Alexander Acevedo De; Hilbert, Ghislaine; Mengin, Virginie; Rivière, Céline; Ollat, Nathalie; Vitrac, Caroline; Bordenave, Louis; Decroocq, Stéphane; Delaunay, Jean‐Claude; Mérillon, Jean‐Michel; Monti, Jean‐Pierre; Gomès, Eric; Richard, Tristan; de, Victor; Comte, Gilles; Santos‐Buelga, Celestino
• Is Part Of: Phytochemical analysis. Volume 24:Issue 5(2013:Sep.)
• Page Start: 446
• Page End: 452
• Publication Date: 2013-07-09
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2447
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <sec id="pca2447-sec-0001" sec-type="section"> <title>Introduction</title> <p>Grapes are one of the most important fruit crops in the world. The quality of red grape berries greatly depends on skin colour, mainly due to the anthocyanin profile. Today, the American <italic>Vitis</italic> species have the greatest potential for breeding work. They have multiple resistance properties in comparison with <italic>Vitis vinifera</italic> but little is known about their anthocyanin content.</p> </sec> <sec id="pca2447-sec-0002" sec-type="section"> <title>Objective</title> <p>To determine the anti‐oxidant properties and anthocyanin profile of two American species, <italic>Vitis candicans</italic> and <italic>Vitis doaniana</italic>, by using LC–MS<italic><sup>n</sup></italic> and LC–NMR.</p> </sec> <sec id="pca2447-sec-0003" sec-type="section"> <title>Methods</title> <p>Grape extracts were prepared by extraction of berry skins with acidified methanol. The complete structure elucidation of the individual anthocyanins was performed with LC–MS<sup>n</sup>, LC–NMR and NMR experiments. Individual anthocyanins in the extracts were quantified by using malvidin glucoside as external standard. The anti‐oxidant activities of grape skin extracts were evaluated by using 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH<sup></sup>) radical scavenging and oxygen radical absorbance capacity (ORAC) assays.</p> </sec> <sec id="pca2447-sec-0004" sec-type="section"> <title>Results</title> <p>By using LC–MS<italic><sup>n</sup></italic> and LC–NMR experiments, 30 anthocyanins were identified and quantified in the two <italic>Vitis</italic> species, including two new <italic>cis‐p</italic>‐coumaroyl derivatives. <italic>Vitis candicans</italic> and <italic>V. doaniana</italic> showed significant differences in their anthocyanin profile. These two <italic>Vitis</italic> species possess low‐to‐medium anti‐oxidant activities in comparison with <italic>V. vinifera</italic>.</p> </sec> <sec id="pca2447-sec-0005" sec-type="section"> <title>Conclusion</title> <p>The profiles of 30 anthocyanins were established unambiguously in two American <italic>Vitis</italic> species. Copyright © 2013 John Wiley & Sons, Ltd.</p> </sec> </abstract>