Mechanical compressive force inhibits adipogenesis of adipose stem cells. (29th August 2013)

• Record Type: Journal Article
• Authors: Li, G.; Fu, N.; Yang, X.; Li, M.; Ba, K.; Wei, X.; Fu, Y.; Yao, Y.; Cai, X.; Lin, Y.
• Is Part Of: Cell proliferation. Volume 46:Number 5(2013:Oct.)
• Page Start: 586
• Page End: 594
• Publication Date: 2013-08-29
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12053
• Languages: English
• ISSNs: 0960-7722

Abstract

<abstract abstract-type="main" id="cpr12053-abs-0001"> <title>Abstract</title> <sec id="cpr12053-sec-0001" sec-type="section"> <title>Objective</title> <p>The aim of this study was to investigate effects of mechanical compressive force on differentiation of adipose‐derived stem cells (ASCs) <italic>in vitro</italic>.</p> </sec> <sec id="cpr12053-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>Mice ASCs were treated with compressive force (2000 με, 1 Hz) for 2 or 6 h after adipogenic induction for 3 days, then oil red O staining was used to examine oil droplet‐filled cells. Adipogenic genes, <italic>PPAR‐γ1</italic> and <italic>APN</italic>, were examined by real‐time PCR and immunofluorescence (IF) staining was performed to test expression of de‐PPAR‐γ and ph‐PPAR‐γ at the protein level.</p> </sec> <sec id="cpr12053-sec-0003" sec-type="section"> <title>Results</title> <p>Our data showed that mechanical compressive force reduced numbers of oil droplet‐filled cells, and down‐regulated mRNA levels of both PPAR‐γ1 and APN and protein level of PPAR‐γ, in ASCs.</p> </sec> <sec id="cpr12053-sec-0004" sec-type="section"> <title>Conclusions</title> <p>In culture medium containing adipogenic stimuli, mechanical compressive force inhibited adipogenesis of ASCs.</p> </sec> </abstract>