Genetic variants in the IL12B gene are associated with inflammatory bowel diseases in the Korean population. Issue 10 (23rd September 2013)

• Record Type: Journal Article
• Authors: Moon, Chang Mo; Shin, Dong‐Jik; Son, Nak‐Hoon; Shin, Eun‐Soon; Hong, Sung Pil; Kim, Tae Il; Kim, Won Ho; Cheon, Jae Hee
• Is Part Of: Journal of gastroenterology and hepatology. Volume 28:Issue 10(2013:Oct.)
• Page Start: 1588
• Page End: 1594
• Publication Date: 2013-09-23
• Subjects: Gastroenterology -- Periodicals; Digestive organs -- Diseases -- Periodicals; Liver -- Diseases -- Periodicals; Gastroenterology -- Periodicals; Liver Diseases -- Periodicals; 616.33
• DOI: 10.1111/jgh.12214
• Languages: English
• ISSNs: 0815-9319

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jgh12214-sec-0001" sec-type="section"> <title>Background and Aim</title> <p>Recent genomic studies have identified genetic variants in the <italic>I</italic><italic>L12B</italic> gene, which encodes the p40 subunit shared by interleukin 12 and interleukin 23, as susceptibility loci for inflammatory bowel disease (IBD). The study aimed to identify additional novel genetic variants in <italic>IL12B</italic> and investigated whether variants confer susceptibility to the development of Crohn's disease (CD) or ulcerative colitis (UC) in the Korean population.</p> </sec> <sec id="jgh12214-sec-0002" sec-type="section"> <title>Methods</title> <p>To detect single nucleotide polymorphisms (SNPs) in <italic>IL12B</italic>, direct sequencing of all coding exons, exon‐intron boundaries, promoter region, and 5′ untranslated region was performed in 24 randomly selected samples. Selected haplotype‐tagging SNPs were subsequently genotyped in 493 IBD patients (245 patients with CD and 248 with UC) and 504 healthy controls.</p> </sec> <sec id="jgh12214-sec-0003" sec-type="section"> <title>Results</title> <p>Two haplotype‐tagging SNPs (rs2288831 and rs919766) were selected through direct sequencing and were genotyped. Of them, SNP rs2288831 in the <italic>IL12B</italic> gene was significantly associated with CD susceptibility in allelic association analysis (odds ratio = 1.30; 95% confidence interval 1.04–1.62; <italic>P</italic> = 0.019). This significant association with CD was also observed for a haplotype consisting of SNP rs919766 and rs2288831 (odds ratio = 1.29; 95% confidence interval 1.03–1.60; <italic>P</italic> = 0.025). However, none of <italic>IL12B</italic> SNPs were associated with UC susceptibility. Finally, no specific associations between genetic variants and disease phenotype of CD were identified.</p> </sec> <sec id="jgh12214-sec-0004" sec-type="section"> <title>Conclusions</title> <p>This study is first to identify SNP rs2288831 in the <italic>IL12B</italic> gene as a susceptible variation for CD. Further studies in other ethnic groups are warranted to validate the association of this genetic variant with IBD.</p> </sec> </abstract>