Identification of three novel human leukocyte antigen alleles, HLA‐B*58:43, HLA‐C*03:190, and HLA‐DPA1*01:12, in an East African cohort. Issue 2 (12th July 2013)

• Record Type: Journal Article
• Authors: Nykoluk, M.; Bailey, R. C.; Moses, S.; Plummer, F. A.; Luo, M.
• Is Part Of: Tissue antigens. Volume 82:Issue 2(2013)
• Page Start: 131
• Page End: 133
• Publication Date: 2013-07-12
• Subjects: Antigens -- Periodicals; Immunological tolerance -- Periodicals; Immunogenetics -- Periodicals; 571.9645
• DOI: 10.1111/tan.12157
• Languages: English
• ISSNs: 0001-2815

Abstract

<abstract abstract-type="main" id="tan12157-abs-0001"> <title>Abstract</title> <p id="tan12157-para-0001">Three novel human leukocyte antigen (HLA) alleles were identified using a sequence‐based typing of HLA class I and class II alleles of 1867 participants from a male circumcision cohort in Kenya. The new alleles were first identified by sequencing and then confirmed by cloning the polymerase chain reaction (PCR) products and sequencing multiple clones. <italic>HLA‐B*58:43</italic> was identical to <italic>HLA‐B*58:02</italic> with the exception of a nucleotide change at codon 125 in exon 3 (GCC→ACC), and resulted in the amino acid change from Alanine to Threonine. <italic>HLA‐C*03:190</italic> was identical to <italic>HLA‐C*03:02:01</italic> with the exception of a nucleotide change at codon 131 in exon 3 (CGC→TGC), and resulted in the amino acid change from Arginine to Cysteine. <italic>HLA‐DPA1*01:12</italic> was identical to <italic>HLA‐DPA1*01:03:01:01</italic> with the exception of a nucleotide change at codon 66 in exon 2 (TTG→TCG), and resulted in the amino acid change from Leucine to Serine.</p> </abstract>