Markers of lipid peroxidation and α‐tocopherol levels in the blood and neoplastic tissue of dogs with malignant mammary gland tumors. (30th July 2013)

• Record Type: Journal Article
• Authors: Karayannopoulou, Maria; Fytianou, Anna; Assaloumidis, Nikolaos; Psalla, Dimitra; Constantinidis, Theodoros C.; Kaldrymidou, Eleni; Koutinas, Alexander F.
• Is Part Of: Veterinary clinical pathology. Volume 42:Number 3(2013)
• Page Start: 323
• Page End: 328
• Publication Date: 2013-07-30
• Subjects: Veterinary pathology -- Periodicals; 636.089607
• DOI: 10.1111/vcp.12064
• Languages: English
• ISSNs: 0275-6382

Abstract

<abstract abstract-type="main" id="vcp12064-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="vcp12064-sec-0001" sec-type="section"> <title>Background</title> <p>Excess reactive oxygen species due to oxidative stress and the ensuing lipid peroxidation are believed to be involved in mammary gland tumor (MGT) pathogenesis.</p> </sec> <sec id="vcp12064-sec-0002" sec-type="section"> <title>Objective</title> <p>The purpose of this study was to evaluate the extent of lipid peroxidation as evidenced by thiobarbituric acid‐reactive substances (TBARS) formation, and the concentration of α‐tocopherol as an inhibitor of lipid peroxidation, in blood and neoplastic tissue of dogs with malignant MGT. The correlation between inflammatory cell infiltration score and TBARS or α‐tocopherol in MGT was also evaluated.</p> </sec> <sec id="vcp12064-sec-0003" sec-type="section"> <title>Methods</title> <p>Sixteen intact female dogs with malignant MGT and 12 clinically healthy and age/weight‐matched controls were included in the study. In all dogs, serum TBARS, α‐tocopherol, total cholesterol, and triglycerides were measured. Tissue TBARS and α‐tocopherol levels were determined in 1 cm<sup>3</sup> sized tissue samples collected from MGT and adjacent, ipsilateral, normal mammary gland tissue from the 16 affected dogs. The degree of inflammatory cell tumor infiltration was evaluated histologically.</p> </sec> <sec id="vcp12064-sec-0004" sec-type="section"> <title>Results</title> <p>There were no statistically significant differences in serum levels of TBARS, α‐tocopherol, total cholesterol, and triglycerides between dogs with and without malignant MGT. TBARS were significantly higher, whereas α‐tocopherol was lower in neoplastic tissue when compared with normal mammary gland tissue. There was no correlation between TBARS or α‐tocopherol concentration and the inflammatory cell infiltration score in neoplastic tissue.</p> </sec> <sec id="vcp12064-sec-0005" sec-type="section"> <title>Conclusion</title> <p>The increased level of TBARS suggests oxidative stress induction in canine malignant MGT. The origin of this phenomenon is not clear, as a potential oxidative burst could not be attributed to inflammatory cells infiltrating the tumors.</p> </sec> </abstract>