Interval Multivariate Curve Resolution in the Dereplication of HPLC–DAD Data from Jatropha gossypifolia. Issue 4 (10th March 2013)

• Record Type: Journal Article
• Authors: Pilon, Alan Cesar; Carneiro, R. L.; Carnevale Neto, F.; da S. Bolzani, Vanderlan; Castro‐Gamboa, I.
• Is Part Of: Phytochemical analysis. Volume 24:Issue 4(2013:Jul.)
• Page Start: 401
• Page End: 406
• Publication Date: 2013-03-10
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2423
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main" id="pca2423-abs-0001"> <title>ABSTRACT</title> <sec id="pca2423-sec-0001" sec-type="section"> <title>Introduction</title> <p> <italic>Jatropha gossypifolia</italic> has been used quite extensively by traditional medicine for the treatment of several diseases in South America and Africa. This medicinal plant has therapeutic potential as a phytomedicine and therefore the establishment of innovative analytical methods to characterise their active components is crucial to the future development of a quality product.</p> </sec> <sec id="pca2423-sec-0002" sec-type="section"> <title>Objective</title> <p>To enhance the chromatographic resolution of HPLC–UV–diode‐array detector (DAD) experiments applying chemometric tools.</p> </sec> <sec id="pca2423-sec-0003" sec-type="section"> <title>Methods</title> <p>Crude leave extracts from <italic>J. gossypifolia</italic> were analysed by HPLC–DAD. A chromatographic band deconvolution method was designed and applied using interval multivariate curve resolution by alternating least squares (MCR–ALS).</p> </sec> <sec id="pca2423-sec-0004" sec-type="section"> <title>Results</title> <p>The MCR–ALS method allowed the deconvolution from up to 117% more bands, compared with the original HPLC–DAD experiments, even in regions where the UV spectra showed high similarity. The method assisted in the dereplication of three <italic>C</italic>‐glycosylflavones isomers: vitexin/isovitexin, orientin/homorientin and schaftoside/isoschaftoside.</p> </sec> <sec id="pca2423-sec-0005" sec-type="section"> <title>Conclusion</title> <p>The MCR–ALS method is shown to be a powerful tool to solve problems of chromatographic band overlapping from complex mixtures such as natural crude samples. Copyright © 2013 John Wiley & Sons, Ltd.</p> </sec> </abstract>