Proteomic analysis of the supernatant of red blood cell units: the effects of storage and leucoreduction. Issue 3 (11th May 2013)

• Record Type: Journal Article
• Authors: Dzieciatkowska, M.; Silliman, C. C.; Moore, E. E.; Kelher, M. R.; Banerjee, A.; Land, K. J.; Ellison, M.; West, F. B.; Ambruso, D. R.; Hansen, K. C.
• Is Part Of: Vox sanguinis. Volume 105:Issue 3(2013)
• Page Start: 210
• Page End: 218
• Publication Date: 2013-05-11
• Subjects: Blood -- Periodicals; Blood -- Transfusion -- Periodicals; Immunohematology -- Periodicals; Immunopathology -- Periodicals; 615.39
• DOI: 10.1111/vox.12042
• Languages: English
• ISSNs: 0042-9007

Abstract

<abstract abstract-type="main" id="vox12042-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="vox12042-sec-0001" sec-type="section"> <title>Background</title> <p>Red blood cell (RBC) transfusion is a life‐saving intervention for critically ill patients; however, it has been linked to increased morbidity and mortality. We hypothesize that a number of important proteins accumulate during routine storage of RBCs, which may explain some of the adverse effects seen in transfused patients.</p> </sec> <sec id="vox12042-sec-0002" sec-type="section"> <title>Study Design</title> <p>Five RBC units were drawn and divided (half prestorage leucoreduced (LR‐RBC) and half left as an unmodified control (RBC). The supernatant was separated on days 1 and 42 of storage and proteomic analyses completed with in‐gel tryptic digestion and nano‐liquid chromatography tandem mass spectrometry.</p> </sec> <sec id="vox12042-sec-0003" sec-type="section"> <title>Results</title> <p>In RBC supernatants, 401 proteins were identified: 203 increased with storage, 114 decreased, and 84 were unchanged. In LR‐RBC supernatant, 231 proteins were identified: 84 increased with storage, 30 decreased, and 117 were unchanged. Prestorage leucoreduction removed many platelet‐ and leucocyte‐derived structural proteins; however, a number of intracellular proteins accumulated including peroxiredoxins (Prdx) 6 and latexin. The increases were confirmed by immunoblotting, including the T‐phosphorylation of Prdx‐6, indicating that it may be functioning as an active phospholipase. Active matrix metalloproteinase‐9 also increased with a coinciding decrease in the metalloproteinase inhibitor 1 and cystatin C.</p> </sec> <sec id="vox12042-sec-0004" sec-type="section"> <title>Conclusion</title> <p>We conclude that a number of proteins increase with RBC storage, which is partially ameliorated with leucoreduction, and transfusion of stored RBCs may introduce mediators that result in adverse events in the transfused host.</p> </sec> </abstract>