Location and characterization of female germline stem cells (FGSCs) in juvenile porcine ovary. (29th August 2013)

• Record Type: Journal Article
• Authors: Bai, Y.; Yu, M.; Hu, Y.; Qiu, P.; Liu, W.; Zheng, W.; Peng, S.; Hua, J.
• Is Part Of: Cell proliferation. Volume 46:Number 5(2013:Oct.)
• Page Start: 516
• Page End: 528
• Publication Date: 2013-08-29
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12058
• Languages: English
• ISSNs: 0960-7722

Abstract

<abstract abstract-type="main" id="cpr12058-abs-0001"> <title>Abstract</title> <sec id="cpr12058-sec-0001" sec-type="section"> <title>Objectives</title> <p>Existence of germline stem cells (GSCs) in juvenile mammalian female ovaries has been drastically debated recently since reports that adult mouse ovaries still have mitotically active germ cells have been proposed. In addition, definitive location of such female germline stem cells (FGSCs) had not been demonstrated.</p> </sec> <sec id="cpr12058-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>We segregated porcine FGSCs mechanically from ovary cortex, and tested our hypotheses by utilizing immunofluorescent staining, qRT‐PCR and western blotting.</p> </sec> <sec id="cpr12058-sec-0003" sec-type="section"> <title>Results</title> <p>We attached emphasis to unambiguous location of FGSCs, which settle simultaneously in the theca. Dissected cells from porcine thecal layers maintained similar characteristics to mouse FGSCs and ESCs over 4‐months <italic>in vitro</italic> culture.</p> </sec> <sec id="cpr12058-sec-0004" sec-type="section"> <title>Conclusion</title> <p>These results may provide a new resource for the study of oogenesis and therapy for ovarian sterility.</p> </sec> </abstract>