P450‐aromatase mRNA is Expressed in the Corpus Luteum (CL) of the Non‐Pregnant Sheep and Goat: The Expression of the Enzyme is Present Throughout Pregnancy in the Goat CL. Issue 1 (21st May 2012)

• Record Type: Journal Article
• Authors: Mondragón, JA; Miranda, C; García‐Mena, J; Ocádiz‐Delgado, R; Gariglio, P; Romano, MC
• Is Part Of: Reproduction in domestic animals. Volume 48:Issue 1(2013)
• Page Start: 85
• Page End: 89
• Publication Date: 2012-05-21
• Subjects: Animal breeding -- Periodicals; Veterinary pathology -- Periodicals; Livestock -- Breeding -- Periodicals; 636.082
• DOI: 10.1111/j.1439-0531.2012.02035.x
• Languages: English
• ISSNs: 0936-6768

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Contents</title> <p>In most mammals, the corpus luteum (CL) and placenta are the major sources of progesterone. The goat pregnancy depends on the presence of CL after mid‐gestation, while sheep pregnancy does not. The expression and distribution of P450‐aromatase (P450‐Aro) mRNA throughout gestation has not been investigated in the goat CL and partially in the sheep CL. The present research was designed to characterize the expression of P450‐Aro mRNA in small ruminant CL with emphasis in the goat. For this purpose, ovaries from Criollo goats and Pelibuey sheeps were analysed using <italic>in situ</italic> reverse transcription‐polymerase chain reaction (RT‐PCR) for the histological detection of P450‐Aro transcripts. In addition, P450‐Aro expression was determined by <italic>in vitro</italic> RT‐PCR. <italic>In situ</italic> RT‐PCR studies showed that the goat and sheep CL were rich in cells positive for P450‐Aro mRNA. We have also found <italic>in vitro</italic> RT‐PCR expression of P450‐Aro mRNA in goat CL at 1, 3 and 4 months of gestation. This study shows that the goat CL expresses P450‐Aro mRNA along gestation, suggesting that this structure is capable to produce oestrogens up to the end of gestation.</p> </abstract>