Analysis of Mogroside V in Siraitia grosvenorii with Micelle‐mediated Cloud‐Point Extraction. Issue 4 (24th January 2013) - Deng, Fuli
ABSTRACT Introduction Mogroside V is the main effective ingredient of Siraitia grosvenorii used as a natural sweet food as well as a traditional Chinese medicine. The sample pre‐treatment prior to chromatographic analysis requires large amounts of toxic organic solvents and is time consuming. Objective To develop an effective and simple method for extracting and determining mogroside V of Siraitia grosvenorii. Methods Mogroside V was extracted and preconcentrated by micelle‐mediated cloud‐point extraction with nonionic surfactant isotridecyl poly (ethylene glycol) ether (Genapol® X‐080). The obtained solutions containing mogroside V were analysed by high‐performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The chromatographic separation was performed on a C18‐column using gradient elution with acetonitrile and water at 203 nm. Results The cloud‐point extraction yield was 80.7% while the pre‐concentration factor was about 10.8. The limit of detection was 0.75 µg/mL and the limit of quantification was 2 µg/mL. The relative standard deviations for intra‐ and interday precisions of mogroside V were less than 8.68% and 5.78%, respectively, and the recoveries were between 85.1% and 103.6%. Conclusion The HPLC–UV method based on micelle‐mediated cloud‐point extraction for determination mogroside V in Siraitia grosvenorii was environmentally friendly, simple and sensitive. Copyright © 2013 John Wiley & Sons, Ltd.