Antibodies to cannabinoid type 1 receptor co‐react with stomatin‐like protein 2 in mouse brain mitochondria. Issue 3 (26th April 2013)

• Record Type: Journal Article
• Authors: Morozov, Yury M.; Dominguez, Martin H.; Varela, Luis; Shanabrough, Marya; Koch, Marco; Horvath, Tamas L.; Rakic, Pasko
• Is Part Of: European journal of neuroscience. Volume 38:Issue 3(2013:Aug.)
• Page Start: 2341
• Page End: 2348
• Publication Date: 2013-04-26
• Subjects: Nervous system -- Periodicals; 612.8
• DOI: 10.1111/ejn.12237
• Languages: English
• ISSNs: 0953-816X

Abstract

<abstract abstract-type="main" id="ejn12237-abs-0001"> <title>Abstract</title> <p>Anti‐cannabinoid type 1 receptor (CB₁) polyclonal antibodies are widely used to detect the presence of CB₁ in a variety of brain cells and their organelles, including neuronal mitochondria. Surprisingly, we found that anti‐CB₁ sera, in parallel with CB₁, also recognize the mitochondrial protein stomatin‐like protein 2. In addition, we show that the previously reported effect of synthetic cannabinoid WIN 55, 212‐2 on mitochondrial complex III respiration is not detectable in purified mitochondrial preparations. Thus, our study indicates that a direct relationship between endocannabinoid signaling and mitochondrial functions in the cerebral cortex seems unlikely, and that caution should be taken interpreting findings obtained using anti‐CB₁ antibodies.</p> </abstract>