Compartmentalization of pro-inflammatory cytokine levels in renal-transplanted pregnant women. (October 2013)

• Record Type: Journal Article
• Authors: Correa-Silva, Simone; Prado, Karen Matias do; Oliveira, Leandro Gustavo; Ono, Erica; Camara, Niels Olsen Saraiva; Bevilacqua, Estela
• Is Part Of: Journal of maternal-fetal & neonatal medicine. Volume 26:Number 15(2013)
• Page Start: 1468
• Page End: 1473
• Publication Date: 2013-10
• Subjects: Obstetrics -- Periodicals; Perinatology -- Periodicals; Infants (Newborn) -- Diseases -- Periodicals; Neonatology -- Periodicals; 618.2
• DOI: 10.3109/14767058.2013.784736
• Languages: English
• ISSNs: 1476-7058

Abstract

<abstract> <title>Abstract</title> <p> <italic>Objective</italic>: We evaluated whether chronic exposure to immunosuppression in transplant recipients modulate the placental inflammatory cytokine levels associated to gestational tolerance mechanisms.</p> <p> <italic>Methods</italic>: Serum samples were collected from 12 renal transplanted pregnant under immunosuppressive regimen treatment and 10 healthy women in second/third trimester of gestation. Term placental tissues (decidua and chorionic villi) were also obtained after elective caesarean. Serum IL-1β, IL-6, IL-8, IL-12p70 and TNF-α were measured, as also in placental homogenates, by Cytometric Bead Array (CBA) combined with flow cytometry and, TGF-β and IL-18 were measured by ELISA.</p> <p> <italic>Results</italic>: Serum levels of IL-6 (<italic>p</italic> = 0.0001) and TNF-α (0.0112) were higher in the 2nd and 3rd trimesters and in decidua the spectrum of increased pro inflammatory cytokines was wider: IL-1β (<italic>p</italic> = 0.0001), IL-6 (<italic>p</italic> = 0.0001), IL-8 (<italic>p</italic> = 0.0001), IL-12p70 (<italic>p</italic> = 0.0001), TGF-β (<italic>p</italic> = 0.0089) and TNF-α (<italic>p</italic> = 0.0002). TGF-β1 was particularly increased in decidual compartment (<italic>p</italic> = 0.001). In the chorionic villous, pro inflammatory profile also were maintained. High IL-1β (<italic>p</italic> = 0.0001), IL-6 (<italic>p</italic> = 0.0001), IL-8 (<italic>p</italic> = 0.0001) and TNF-α (<italic>p</italic> = 0.0001) levels establish a similar pattern to that seem in decidua.</p> <p> <italic>Conclusion</italic>: Immunosuppressors may impair the immune response, but when associated with pregnancy the cytokine levels seems to shift a proinflammatory profile in placental compartments, which might also impact on the gestational outcomes in transplanted mothers.</p> </abstract>