The ATP‐binding Cassette Transporter OsABCG15 is Required for Anther Development and Pollen Fertility in Rice. Issue 8 (17th June 2013)

• Record Type: Journal Article
• Authors: Niu, Bai‐Xiao; He, Fu‐Rong; He, Ming; Ren, Ding; Chen, Le‐Tian; Liu, Yao‐Guang
• Is Part Of: Journal of integrative plant biology. Volume 55:Issue 8(2013)
• Page Start: 710
• Page End: 720
• Publication Date: 2013-06-17
• Subjects: Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.12053
• Languages: English
• ISSNs: 1672-9072

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="jipb12053-sec-0001" sec-type="section"> <p>Plant male reproductive development is a complex biological process, but the underlying mechanism is not well understood. Here, we characterized a rice (<italic>Oryza sativa</italic> L.) male sterile mutant. Based on map‐based cloning and sequence analysis, we identified a 1, 459‐bp deletion in an adenosine triphosphate (ATP)‐binding cassette (ABC) transporter gene, <italic>OsABCG15</italic>, causing abnormal anthers and male sterility. Therefore, we named this mutant <italic>osabcg15</italic>. Expression analysis showed that <italic>OsABCG15</italic> is expressed specifically in developmental anthers from stage 8 (meiosis II stage) to stage 10 (late microspore stage). Two genes <italic>CYP704B2</italic> and <italic>WDA1</italic>, involved in the biosynthesis of very‐long‐chain fatty acids for the establishment of the anther cuticle and pollen exine, were downregulated in <italic>osabcg15</italic> mutant, suggesting that <italic>OsABCG15</italic> may play a key function in the processes related to sporopollenin biosynthesis or sporopollenin transfer from tapetal cells to anther locules. Consistently, histological analysis showed that <italic>osabcg15</italic> mutants developed obvious abnormality in postmeiotic tapetum degeneration, leading to rapid degredation of young microspores. The results suggest that <italic>OsABCG15</italic> plays a critical role in exine formation and pollen development, similar to the homologous gene of <italic>AtABCG26</italic> in <italic>Arabidopsis</italic>. This work is helpful to understand the regulatory network in rice anther development.</p> </sec> </abstract>