Trans‐vessel gradient of myeloperoxidase in coronary artery disease. (22nd July 2013)

• Record Type: Journal Article
• Authors: Alipour, Arash; Ribalta, Josep; Njo, Tjin L.; Janssen, Hans W.; Birnie, Erwin; van, Addy J. M.; Elte, Jan Willem F.; Castro Cabezas, Manuel
• Is Part Of: European journal of clinical investigation. Volume 43:Number 9(2013:Sep.)
• Page Start: 920
• Page End: 925
• Publication Date: 2013-07-22
• Subjects: Pathology -- Periodicals; Medical research -- Periodicals; 616.075
• DOI: 10.1111/eci.12121
• Languages: English
• ISSNs: 0014-2972

Abstract

<abstract abstract-type="main" id="eci12121-abs-0001"> <title>Abstract</title> <sec id="eci12121-sec-0001" sec-type="section"> <title>Background</title> <p>Coronary artery disease (CAD) may reflect generalized inflammation. We evaluated leucocyte activation in subjects with and without CAD in different vascular compartments.</p> </sec> <sec id="eci12121-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>Patients were divided in two groups; subjects without CAD (controls; <italic>n </italic>= 25) and with stable CAD (<italic>n </italic>= 52) based on coronary angiography. After blood sampling from vessels, cardiovascular risk factors and leucocyte activation markers CD11b, CD66b and cytoplasmatic myeloperoxidase (MPO) were determined by flow cytometry.</p> </sec> <sec id="eci12121-sec-0003" sec-type="section"> <title>Results</title> <p>Myeloperoxidase (MPO) was higher in patients with CAD at all sites compared with controls (188 ± 7 vs. 210 ± 12 au for venous (<italic>P </italic>< 0·05), 178 ± 7 vs. 212 ± 12 au for femoral artery (<italic>P </italic>= 0·08), 166 ± 7 vs. 195 ± 12 au for abdominal artery (<italic>P </italic>< 0·05), 166 ± 6 vs. 189 ± 14 au for left coronary (<italic>P </italic>= 0·08) and 163 ± 6 vs. 193 ± 12 au for the right coronary artery (<italic>P </italic>< 0·05)). Other markers did not differ between the groups. A gradient of inflammation from peripheral vessels to the coronaries was found by differences in MPO in both groups; from 210 ± 12 au in the venous compartments towards 189 ± 14 and 193 ± 12 au, in the left and right coronaries, respectively, for the controls (<italic>P </italic>= 0·001), and from 188 ± 7 au in the venous compartment towards 166 ± 6 and 163 ± 6 au in the left and right coronaries, respectively, for the patients (<italic>P </italic>= 0·007). Other leucocyte activation markers did not show such a gradient.</p> </sec> <sec id="eci12121-sec-0004" sec-type="section"> <title>Conclusions</title> <p>There is a generalized inflammatory neutrophil gradient for MPO from peripheral vessels towards the coronaries in both patients with CAD and controls. However, patients with CAD show a higher degree of inflammation, mostly in the coronaries. These data strengthen the role of activated neutrophils in CAD.</p> </sec> </abstract>