Premature senescence and cellular phenotype transformation of mesangial cells induced by TGF-B1. (September 2013)

• Record Type: Journal Article
• Authors: Fu, Rong-Guo; Wu, Juan-Juan; Xue, Rong-Liang; Zhang, Tao; Wang, Li; Wu, Xi-Li; Duan, Zhao-Yang; Jia, Li-Ning; Ma, Li-Qun; Du, Yan; Wei, Lin-Ting
• Is Part Of: Renal failure. Volume 35:Number 8(2013)
• Page Start: 1142
• Page End: 1145
• Publication Date: 2013-09
• Subjects: Chronic renal failure -- Periodicals; Acute renal failure -- Periodicals; Uremia -- Periodicals; 616.614005
• DOI: 10.3109/0886022X.2013.817279
• Languages: English
• ISSNs: 0886-022X

Abstract

<abstract> <title>Abstract</title> <p> <italic>Background</italic>: Transforming growth factor-β1 (TGF-β1) is a polypeptide member of the transforming growth factor β superfamily of cytokines and performs many cellular functions. Its overexpression may lead to renal fibrosis. <italic>Aim</italic>: This study planed to investigate the effects of TGF-β1 on the cell cycle and phenotype of mesangial cells. <italic>Methods</italic>: Rat mesangial cells were cultured together with different concentrations (0, 1, 2, 5, and 10 ng/mL) of TGF-β1 for specified times from 0 min to 72 h. 0 ng/mL TGF-β1 and 0 min served as controls. Cell cycles were assessed by flow cytometry and α-smooth muscle actin expression (α-SMA) protein expression by western blot analysis. All data were presented as Mean ± SD. Statistical analysis was performed by using one-way analysis of variance and correlation analysis. Results were considered significant at <italic>p < </italic>0.05. <italic>Results</italic>: After 15 min of co-culture with different concentrations of TGF-β1, the percentage of mesangial cells in G0/G1 phase was significantly elevated compared to the control (<italic>p < </italic>0.05). 12 h co-culture induced cell hyperplasia, 24 h co-culture obvious up-regulation of α-SMA (<italic>p < </italic>0.01) and one or two cells' myofibroblast phenotype transition, and 36 h co-culture several cells' phenotype transition. Correlation analysis prompted that the TGF-β1-induced premature aging was time-dependent (<italic>p < </italic>0.01). <italic>Conclusion</italic>: TGF-β1 may induce mesangial cells' premature senescence and myofibroblast-like phenotype transformation time-dependently, which may contribute to the development of early stage of glomerulosclerosis.</p> </abstract>