HPV‐genotypes in high‐grade intraepithelial cervical lesions in Danish women. (1st June 2013)

• Record Type: Journal Article
• Authors: Kirschner, Benny; Schledermann, Doris; Holl, Katsiaryna; Rosenlund, Mats; Raillard, Alice; Quint, Wim; Molijn, Anco; Jenkins, David; Junge, Jette
• Is Part Of: Acta obstetricia et gynecologica Scandinavica. Volume 92:Number 9(2013)
• Page Start: 1032
• Page End: 1040
• Publication Date: 2013-06-01
• Subjects: Gynecology -- Periodicals; Pregnancy -- Periodicals; Obstetrics -- Periodicals; 618.05
• DOI: 10.1111/aogs.12162
• Languages: English
• ISSNs: 0001-6349

Abstract

<abstract abstract-type="main" id="aogs12162-abs-0001"> <title>Abstract</title> <sec id="aogs12162-sec-0001" sec-type="section"> <title>Objective</title> <p>A study was undertaken to assess the distribution of high‐risk HPV‐genotypes in high‐grade cervical intraepithelial neoplastic lesions in Danish women.</p> </sec> <sec id="aogs12162-sec-0002" sec-type="section"> <title>Design</title> <p>Observational, cross‐sectional.</p> </sec> <sec id="aogs12162-sec-0003" sec-type="section"> <title>Setting</title> <p>Danish data from a multi‐centre study undertaken in 13 European countries.</p> </sec> <sec id="aogs12162-sec-0004" sec-type="section"> <title>Population</title> <p>290 archived fixed biopsies with high‐grade cervical lesions from the Departments of Pathology at the University Hospitals in Hvidovre and Odense, Denmark.</p> </sec> <sec id="aogs12162-sec-0005" sec-type="section"> <title>Methods</title> <p>Relevant histological samples were anonymized and shipped to a central laboratory for histopathology review and PCR‐testing for HPV‐DNA. A standardised HPV‐test methodology was utilised to enable comparison of HPV‐genotype distribution.</p> </sec> <sec id="aogs12162-sec-0006" sec-type="section"> <title>Results</title> <p>Of 290 Danish cervical samples, 276 were evaluated as histologically adequate and all of these were HPV‐positive (HPV<sup>+</sup>). Of the HPV<sup>+</sup> samples 77.9% were diagnosed with a single HPV‐type, with cervical intraepithelial neoplasia (CIN)3 diagnosed in 82.3% and CIN2, CIN2/3, adenocarcinoma in situ (AIS) and AIS<sup>+</sup> other high‐grade lesion diagnosed in the remaining 17.7%. The most prevalent HPV‐types were: HPV16 (54.0%), HPV33 (13.5%), HPV31 (10.7%), HPV18 (7.9%) and HPV52 (4.7%). Of the HPV<sup>+</sup> samples, 21.4% were diagnosed with multiple HPV‐types, with CIN3 diagnosed in 79.6% and CIN2, CIN2/3, AIS and AIS<sup>+</sup> other high‐grade lesion diagnosed in the remaining 20.4%. The most prevalent HPV‐types were: HPV16 (49.2%), HPV31 (30.5%), HPV52 (27.1%), HPV51 (20.3%), HPV18 (16.9%), HPV33 (13.6%), HPV45 (11.9%), with 0.7% unknown types.</p> </sec> <sec id="aogs12162-sec-0007" sec-type="section"> <title>Conclusions</title> <p>HPV16 and HPV18 were detected in approximately 75% of high‐grade intraepithelial cervical lesions in a Danish population (single or multiple infections); these two genotypes are considered causative in at least 61.9% of the high‐grade intraepithelial lesions (single infection).</p> </sec> </abstract>