Overview of available p53 function tests in relation to TP53 and ATM gene alterations and chemoresistance in chronic lymphocytic leukemia. Issue 8 (August 2013)

• Record Type: Journal Article
• Authors: te Raa, G. Doreen; Malcikova, Jitka; Pospisilova, Sarka; Trbusek, Martin; Mraz, Mark; Garff-Tavernier, Maria Le; Merle-Béral, Hélène; Lin, Ke; Pettitt, Andrew R.; Merkel, Olaf; Stankovic, Tatjana; van Oers, Marinus H.; Eldering, Eric; Stilgenbauer, Stephan; Zenz, Thorsten; Kater, Arnon P.
• Is Part Of: Leukemia & lymphoma. Volume 54:Issue 8(2013:Aug.)
• Page Start: 1849
• Page End: 1853
• Publication Date: 2013-08
• Subjects: Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.3109/10428194.2013.796058
• Languages: English
• ISSNs: 1042-8194

Abstract

<abstract> <title>Abstract</title> <p>The ATM–p53 DNA damage response pathway plays a crucial role in chemoresistance in chronic lymphocytic leukemia, as indicated by the adverse prognostic impact of deletions of 17p (locus of <italic>TP53</italic>) and 11q (locus of <italic>ATM</italic>) detected by fluorescence <italic>in situ</italic> hybridization (FISH) analysis. In addition to deletions, mutations in these respective genes are also associated with chemoresistance, and add to the prognostic information provided by FISH. In order to explore the possibility that dysfunction of the ATM–p53 pathway might also result from mechanisms other than <italic>ATM</italic>/<italic>TP53</italic> deletion/mutation, assays have been developed that probe the functional integrity of the ATM–p53 pathway. Currently, four different p53 function assays have been developed that are based on the measurement of p53 and p53-dependent genes at the RNA (real-time polymerase chain reaction [RT-PCR]<italic>p21</italic>; RT-PCR<italic>miR34a</italic>; reverse transcription-multiplex ligation-dependent probe amplification assay [RT-MLPA]<italic>p21, bax, puma</italic> and <italic>CD95</italic>) or protein (fluorescence activated cell sorting [FACS]p53-p21) level in untreated cells or following irradiation or drug treatment. Here we provide an overview of these assays based on the available literature.</p> </abstract>