Evaluation and implementation of a membrane filter method for Cronobacter detection in drinking water. Issue 1 (22nd April 2013)

• Record Type: Journal Article
• Authors: Liu, Hui; Yang, Yuelian; Cui, Jinghua; Liu, Lanzheng; Liu, Huiyuan; Hu, Guangchun; Shi, Yuwen; Li, Jian
• Is Part Of: FEMS microbiology letters. Volume 344:Issue 1(2013:Jul.)
• Page Start: 60
• Page End: 68
• Publication Date: 2013-04-22
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1111/1574-6968.12155
• Languages: English
• ISSNs: 0378-1097

Abstract

<abstract abstract-type="main" id="fml12155-abs-0001"> <title>Abstract</title> <p>A membrane filter (MF) method was evaluated for its suitability for qualitative and quantitative analyses of <italic>Cronobacter</italic> spp. in drinking water by pure strains of <italic>Cronobacter</italic> and non‐<italic>Cronobacter</italic>, and samples spiked with chlorinated <italic>Cronobacter sakazakii</italic> ATCC 29544. The applicability was verified by the tests: for pure strains, the sensitivity and the specificity were both 100%; for spiked samples, the MF method recovered 82.8 ± 10.4% chlorinated ATCC 29544 cells. The MF method was also applied to screen <italic>Cronobacter</italic> spp. in drinking water samples from municipal water supplies on premises (MWSP) and small community water supplies on premises (SCWSP). The isolation rate of <italic>Cronobacter</italic> spp. from SCWSP samples was 31/114, which was significantly higher than that from MWSP samples which was 1/131. Besides, the study confirmed the possibility of using total coliform as an indicator of contamination level of <italic>Cronobacter</italic> spp. in drinking water, and the acquired correct positive rate was 96%.</p> </abstract>