Epilobium hirsutum alters xenobiotic metabolizing CYP1A1, CYP2E1, NQO1 and GPx activities, mRNA and protein levels in rats. (May 2013)

• Record Type: Journal Article
• Authors: Karakurt, Serdar; Semiz, Aslı; Celik, Gurbet; Gencler-Ozkan, Ayşe Mine; Sen, Alaattin; Adali, Orhan
• Is Part Of: Pharmaceutical biology. Volume 51:Number 5(2013:May)
• Page Start: 650
• Page End: 658
• Publication Date: 2013-05
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2012.762404
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Natural products have attracted increasing interests due to their use in flavoring, nutrition, cosmetics, pharmacy and medicine. <italic>Epilobium hirsutum</italic> L. (Onagraceae) is known for its analgesic, antimicrobial, and antiproliferative activity. CYP1A1 and CYP2E1, xenobiotic metabolizing enzymes, serve as a metabolic activation route yielding reactive metabolites that are eliminated by the action of NQO1 and glutathione peroxidase (GPx) enzymes.</p> <p> <italic>Objective:</italic> This study investigated <italic>in vivo</italic> effects of <italic>Epilobium hirsutum</italic> (<italic>EH</italic>) on CYP2E1, CYP1A1, NQO1 and GPx activities, protein and mRNA expressions in liver.</p> <p> <italic>Materials and methods:</italic> Male Wistar Albino rats were injected with <italic>EH</italic> at a dose of 37.5 mg/kg i.p. daily for 9 d. CYP2E1, CYP1A1, NQO1 and GPx activities, protein and mRNA levels were determined by enzyme assays, Western blotting and qPCR, respectively.</p> <p> <italic>Results:</italic> CYP1A1 associated ethoxyresorufin-<italic>O</italic>-deethylase activity of control and <italic>EH</italic>-treated animals were found as 6.54 ± 1.21 and 4.48 ± 1.67 nmol/min/mg, respectively. CYP2E1 associated aniline 4-hydroxylase of control and <italic>EH</italic> group were 0.537 ± 0.011 and 0.109 ± 0.01 nmol/min/mg, respectively. However, <italic>EH</italic> treatment increased the GPx and NQO1 activities from 0.069 ± 0.015 to 0.107 ± 0.026 nmol/min/mg and from 163.34 ± 92 to 588.3 ± 14 nmol/min/mg, respectively. Furthermore, protein and mRNA expression analysis revealed that CYP1A1 and CYP2E1 levels were decreased while those of NQO1 and GPx increased after <italic>EH</italic> treatment.</p> <p> <italic>Discussion and conclusion:</italic> Our current data suggest that the metabolism of xenobiotics, including drugs, may be altered due to changes in the expression and activity of these proteins by <italic>EH</italic>.</p> </abstract>