Chemical debridement of contaminated titanium surfaces: Anin vitrostudy. (May 2013)

• Record Type: Journal Article
• Authors: Henderson, Elisabet; Schneider, Sigurd; Petersen, Fernanda C.; Haugen, Håvard J.; Wohlfahrt, Johan Caspar; Ekstrand, Karl; Ekfeldt, Anders
• Is Part Of: Acta odontologica Scandinavica. Volume 71:Number 3/4(2013)
• Page Start: 957
• Page End: 964
• Publication Date: 2013-05
• Subjects: Dentistry -- Periodicals; 617.6005
• DOI: 10.3109/00016357.2012.734423
• Languages: English
• ISSNs: 0001-6357

Abstract

<abstract> <title>Abstract</title> <p> <bold> <italic>Objective.</italic> </bold> To compare the efficacy of different chemical solutions when used for chemical debridement of biofilm contaminated titanium surfaces in an in-vitro experimental study. <bold><italic>Materials and methods.</italic></bold> Commercially pure titanium discs with a diameter of 6.2 mm and height of 2 mm, mirror-polished with a measured surface amplitude value S<sub>A</sub> = 0.037 μm ± 0.009 were used as test-surfaces. A biofilm was simulated with multi-layers of <italic>Staphylococcus epidermidis</italic> ATCC359844 covering the entire titanium surface. The chemical agents tested were: 3% H<sub>2</sub>O<sub>2</sub>, 0.2% Chlorhexidine, 24% EDTA-gel, 3% H<sub>2</sub>O<sub>2</sub> mixed with 1.6 g/L TiO<sub>2</sub> and sterile saline solution. The decontamination effect was evaluated by optical density analysis using spectrophotometry and with scanning electron microscopy (SEM) images of the remaining biofilm. <bold><italic>Results.</italic></bold> The suspensions of 3% H<sub>2</sub>O<sub>2</sub> and 1.6 g/L TiO<sub>2</sub> or 3% H<sub>2</sub>O<sub>2</sub> alone were the most effective in removing <italic>S. epidermidis</italic> biofilms (<italic>p</italic> < 0.05), whereas 0.2% chlorhexidine or 24% EDTA gel had no significant effects. SEM images of the remaining biofilms supported the quantitative results indicating the higher efficacy of 3% H<sub>2</sub>O<sub>2</sub> and 1.6 g/L TiO<sub>2</sub> or 3% H<sub>2</sub>O<sub>2</sub> alone. It also revealed that EDTA, despite a non-significant effect on reducing the amount of established biofilms, was able to alter the biofilm architecture, as demonstrated by increased interspaced regions. <bold><italic>Conclusions.</italic></bold> In this <italic>in vitro</italic> study the decontamination potential of a suspension of 3% H<sub>2</sub>O<sub>2</sub> and 1.6 g/L TiO<sub>2</sub> or 3% H<sub>2</sub>O<sub>2</sub> alone were encouraging. Whether such procedures would have a similar effect <italic>in vivo</italic> remains to be determined.</p> </abstract>