Genotoxicity of multi-walled carbon nanotubes in both in vitro and in vivo assay systems. Issue 4 (June 2013)

• Record Type: Journal Article
• Authors: Kato, Tatsuya; Totsuka, Yukari; Ishino, Kousuke; Matsumoto, Yoko; Tada, Yukie; Nakae, Dai; Goto, Sumio; Masuda, Shuichi; Ogo, Sayaka; Kawanishi, Masanobu; Yagi, Takashi; Matsuda, Tomonari; Watanabe, Masatoshi; Wakabayashi, Keiji
• Is Part Of: Nanotoxicology. Volume 7:Issue 4(2013:Jun.)
• Page Start: 452
• Page End: 461
• Publication Date: 2013-06
• Subjects: Toxicology -- Periodicals; 615.9
• DOI: 10.3109/17435390.2012.674571
• Languages: English
• ISSNs: 1743-5390

Abstract

<abstract> <title>Abstract</title> <p>The genotoxic effects of multi-walled carbon nanotubes (MWCNTs) were examined by using <italic>in vitro</italic> and <italic>in vivo</italic> assays. MWCNTs significantly induced micronuclei in A549 cells and enhanced the frequency of sister chromatid exchange (SCE) in CHO AA8 cells. When ICR mice were intratracheally instilled with a single dose (0.05 or 0.2 mg/animal) of MWCNTs, DNA damage of the lungs, analysed by comet assay, increased in a dose-dependent manner. Moreover, DNA oxidative damage, indicated by 8-oxo-7, 8-dihydro-2′-deoxyguanosine and heptanone etheno-deoxyribonucleosides, occurred in the lungs of MWCNT-exposed mice. The <italic>gpt</italic> mutation frequencies significantly increased in the lungs of MWCNT-treated <italic>gpt</italic> delta transgenic mice. Transversions were predominant, and G:C to C:G was clearly increased by MWCNTs. Moreover, many regions immunohistochemically stained for inducible NO synthase and nitrotyrosine were observed in the lungs of MWCNT-exposed mice. Overall, MWCNTs were shown to be genotoxic both in <italic>in vitro</italic> and <italic>in vivo</italic> tests; the mechanisms probably involve oxidative stress and inflammatory responses.</p> </abstract>