Dual ligands modified double targeted nano-system for liver targeted gene delivery. (May 2013)

• Record Type: Journal Article
• Authors: Jing, Fanbo; Li, Jing; Liu, Donghua; Wang, Chenjing; Sui, Zhongguo
• Is Part Of: Pharmaceutical biology. Volume 51:Number 5(2013:May)
• Page Start: 643
• Page End: 649
• Publication Date: 2013-05
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2012.761245
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context:</italic> It is now well established that the surface of nanocarriers with specific ligands defines a new biological identity, which assist in targeting and internalization of the nanocarriers to specific cell populations, such as cancers and disease organs.</p> <p> <italic>Objective:</italic> The aim of this study is to develop systemically administrable dual ligands modified nano-system which could both target cancer cells and macrophages in the liver.</p> <p> <italic>Methods:</italic> Transferrin (Tf) and mannan (M) were linked onto polyethylene glycol-phosphatidylethanolamine (PEG-PE) and PE separately to get transferrin-PEG-PE (T-PEG-PE) and mannan-PE (M-PE) ligands for the surface modification of carriers. The <italic>in vivo</italic> transfection efficiency of the novel dual ligands modified (D-modified) vectors were evaluated in tumor bearing animal models.</p> <p> <italic>Results:</italic> D-modified solid lipid nanoparticles/enhanced green fluorescence protein plasmid (D-SLN/pEGFP) has a particle size of 198 nm and a gene loading quantity of 89%. D-SLN/pEGFP displayed over 25% higher transfection efficiency than M-PE modified SLN/pEGFP (M-SLN/pEGFP) in HepG2 cells and T-PEG-PE modified SLN/pEGFP (T-SLN/pEGFP) in Kupffer cells (KCs) isolated from mice.</p> <p> <italic>Conclusion:</italic> It could be concluded that T-PEG-PE and M-PE could function as excellent active targeting ligands to improve the cell targeting ability of the carriers and the dual ligands modified vectors could be applied as a promising active targeting gene delivery system.</p> </abstract>