Fine Mapping of RppP25, a Southern Rust Resistance Gene in Maize12. Issue 5 (11th April 2013)

• Record Type: Journal Article
• Authors: Zhao, Panfeng; Zhang, Guobin; Wu, Xiaojun; Li, Na; Shi, Dianyi; Zhang, Dengfeng; Ji, Chunfang; Xu, Mingliang; Wang, Shoucai
• Is Part Of: Journal of integrative plant biology. Volume 55:Issue 5(2013)
• Page Start: 462
• Page End: 472
• Publication Date: 2013-04-11
• Subjects: Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.12027
• Languages: English
• ISSNs: 1672-9072

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jipb12027-sec-0001" sec-type="section"> <p> <bold>Southern rust (<italic>Puccinia polysora</italic> Underw.) is a major disease that can cause severe yield losses in maize (<italic>Zea mays</italic> L.). In our previous study, a major gene <italic>RppP25</italic> that confers resistance to southern rust was identified in inbred line P25. Here, we report the fine mapping and candidate gene analysis of <italic>RppP25</italic> from the near‐isogenic line F939, which harbors <italic>RppP25</italic> in the genetic background of the susceptible inbred line F349. The inheritance of resistance to southern rust was investigated in the BC<sub>1</sub>F<sub>1</sub> and BC<sub>3</sub>F<sub>1</sub> populations, which were derived from a cross between F939 and F349 (as the recurrent parent). The 1:1 segregation ratio of resistance to susceptible plants in these two populations indicated that the resistance is controlled by a single dominant gene. Ten markers, including three simple sequence repeat (SSR) markers and seven insertion/deletion (InDel) markers, were developed in the <italic>RppP25</italic> region. <italic>RppP25</italic> was delimited to an interval between P091 and M271, with an estimated length of 40 kb based on the physical map of B73. In this region, a candidate gene was identified that was predicted to encode a putative nucleotide‐binding site leucine‐rich repeat (NBS‐LRR) protein. Two co‐segregated markers will aid in pyramiding diverse southern rust resistance alleles into elite materials, and thereby improve southern rust resistance worldwide.</bold> </p> </sec> </abstract>