Genotoxic biomonitoring of flowable and non-flowable composite resins in peripheral blood leukocytes. (May 2013)

• Record Type: Journal Article
• Authors: Tadin, Antonija; Marovic, Danijela; Galic, Nada; Milevoj, Alice; Medvedec Mikic, Ivana; Zeljezic, Davor
• Is Part Of: Acta odontologica Scandinavica. Volume 71:Number 3/4(2013)
• Page Start: 923
• Page End: 929
• Publication Date: 2013-05
• Subjects: Dentistry -- Periodicals; 617.6005
• DOI: 10.3109/00016357.2012.734419
• Languages: English
• ISSNs: 0001-6357

Abstract

<abstract> <title>Abstract</title> <p> <bold> <italic>Objective.</italic> </bold> Composite restorative materials represent one of the most important groups of materials in contemporary dental practice. However, their incomplete polymerization may lead to monomer-induced genotoxicity. The objective of this study was to evaluate the genotoxicity of three flowable (Filtek Supreme XT Flow, Tetric EvoFlow, Gradia Direct Flo) and three non-flowable dental composite materials (Filtek Z250, Tetric EvoCeram, Gradia Direct Posterior). <bold><italic>Materials and methods.</italic></bold> Genotoxicity assessment of composite materials was carried out <italic>in vitro</italic> in human peripheral blood leukocytes using the alkaline single-cell gel electrophoresis technique (comet assay). Prepared materials were eluted in saline solution for 1 h, 1 day and 5 days. Thereafter leukocyte cultures were treated with different concentrations of eluates obtained from each of the tested dental composite materials. Kruskall-Wallis non-parametric test was used for statistical analysis (<italic>p</italic> < 0.05). <bold><italic>Results.</italic></bold> The tested materials did not show genotoxic effects after exposure of leucocytes to 1 h eluates. Culture treated with 1 day eluates of all tested materials, only at a highest concentration (10<sup>−2</sup>), affected the measured cytogenetic parameters. Of all tested materials, only Filtek Z250 and Filtek Supreme XT Flow did not exhibit a genotoxic effect in cultures that were under the influence of 5 day eluates. <bold><italic>Conclusion.</italic></bold> Tested materials exhibited limited genotoxic activity in peripheral blood leukocytes. Since the effect was observed only in leukocyte cultures treated by 1-day eluates at the highest concentration (10<sup>−2</sup>) and it decreases in cultures exposed to 5 day eluates, it should not pose a significant risk to the human genome.</p> </abstract>