Helicobacter pylori free‐living and biofilm modes of growth: behavior in response to different culture media. Issue 6 (12th December 2012)

• Record Type: Journal Article
• Authors: Bessa, Lucinda J.; Grande, Rossella; Iorio, Donato DI; Giulio, Mara DI; Campli, Emanuela DI; Cellini, Luigina
• Is Part Of: Apmis. Volume 121:Issue 6(2013:Jun.)
• Page Start: 549
• Page End: 560
• Publication Date: 2012-12-12
• Subjects: Pathology -- Periodicals; Microbiology -- Periodicals; Immunology -- Periodicals; 572
• DOI: 10.1111/apm.12020
• Languages: English
• ISSNs: 0903-4641

Abstract

<abstract abstract-type="main" id="apm12020-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The physiological behavior of <italic>Helicobacter pylori</italic> in different growth conditions was investigated to approach its growth standardization. <italic>H. pylori</italic> free‐living and biofilm modes of growth were assessed in four different liquid culture media (Brucella broth, brain heart infusion broth and Ham's F‐12 supplemented with 2% fetal calf serum and Ham's F‐12 without serum). Free‐living growth was monitored during 72 h in each medium and characterized for bacterial density, culturability, viability and morphology. The biofilm formation in the same media was evaluated for biomass production, colony forming unit (CFU) counts and microscopic visualization. Afterward, using Ham's F‐12, the effect of amoxicillin and clarithromycin at sub‐ minimum inhibitory concentrations (sub‐MICs) was evaluated on <italic>H. pylori</italic> biofilm formation and <italic>luxS</italic> gene expression. Differences in the free‐living growth were observed between the media supplemented with serum and Ham's F‐12 without serum. Biofilm formation was significantly dependent on the growth media used. Ham's F‐12 seems to be a good medium to support both growth phenotypes of <italic>H. pylori</italic>. Moreover, sub‐MICs of antibiotics increased the biofilm formation and affected the <italic>luxS</italic> gene expression. Optimizing the growth conditions of <italic>H. pylori</italic>, especially in the biofilm mode, will be helpful to perform more accurate in‐depth studies that will allow increasing the knowledge about <italic>H. pylori</italic> biofilm, which should be a target to eradicate resistant infection.</p> </abstract>