A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. (May 2013)

• Record Type: Journal Article
• Authors: Rao, T. Jagan Mohan; Goyal, Arun
• Is Part Of: International journal of food sciences and nutrition. Volume 64:Number 3(2013)
• Page Start: 346
• Page End: 354
• Publication Date: 2013-05
• Subjects: Food -- Analysis -- Periodicals; Nutrition -- Periodicals; 641.3
• DOI: 10.3109/09637486.2012.734289
• Languages: English
• ISSNs: 0963-7486

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <p>A new isolate from apple (<italic>Malus domestica</italic>) producing dextransucrase and dextran was characterized by morphological, biochemical and 16S rDNA analyses. The bacterium that was identified as <italic>Weissella cibaria</italic> (GenBank accession number KC110687) was Gram positive, non-motile, short rod, catalase negative, vancomycin resistant. The maximum dextransucrase activity (5.8 U/ml) as well as dextran concentration of 38 mg/ml was observed at 24°C after 12 h of growth under static condition. The polyethylene glycol (PEG)-400 fractionation of partially purified enzyme showed a specific activity of 20.0 U/mg and displayed a single homogenous band of molecular size 177 kDa on sodium dodecyl sulphate-Poly acrylamide gel electrophoresis (SDS-PAGE) (7.5%). The dextran was enzymatically synthesized using dextransucrase and substrate sucrose. Dextran was purified by alcohol precipitation for further characterization. The rheological analysis of dextran elucidated a non-Newtonian pseudoplastic behaviour. ¹H and ¹³C NMR analyses revealed the fact that the dextran contained <sc>d</sc>-glucose monomer with 93.0% of α(1 → 6) linear and 7.0% of α(1 → 3) branched linkages.</p> </abstract>