The effect of molybdenum on the in vitro development of mouse preimplantation embryos. (April 2013)

• Record Type: Journal Article
• Authors: Bi, Cong-Ming; Zhang, Yu-Ling; Liu, Feng-Jun; Zhou, Tie-Zhong; Yang, Zi-Jun; Gao, Shen-Yang; Wang, Shu-De; Chen, Xiao-Li; Zhai, Xiao-Wei; Ma, Xue-Gang; Jin, Li-Jun; Wang, Shen
• Is Part Of: Systems biology in reproductive medicine. Volume 59:Number 2(2013:Apr.)
• Page Start: 69
• Page End: 73
• Publication Date: 2013-04
• Subjects: Systems biology -- Periodicals; Andrology -- Periodicals; Generative organs, Male -- Diseases -- Periodicals; Biological systems -- Periodicals; Reproductive health -- Periodicals; Human reproduction -- Periodicals; 612.61
• DOI: 10.3109/19396368.2012.743198
• Languages: English
• ISSNs: 1939-6368

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <p>The object of this study was to investigate the effect of molybdenum on the development of mouse preimplantation embryos cultured <italic>in vitro</italic>. Zygotes were flushed from one outbred mouse strain (Kunming), and then were cultured in potassium simplex optimized medium (KSOM) containing 0, 5, 10, 20, 40, 80, 120, and 160 µg/ml of molybdenum for 5 days until the mid-blastocyst stage. The addition of ≤ 20 µg/ml molybdenum did not affect the blastocyst and birth rates. Molybdenum at doses of 40 µg/ml and higher significantly decreased the cleavage, blastocyst and birth rates, the average cell number, and significantly increased the proportion of degenerative blastocysts. At 120 µg/ml molybdenum inhibited the blastocysts development to birth. At 160 µg/ml molybdenum caused overall developmental arrest (up to 16-cells) of embryos and their massive degeneration. In conclusion, molybdenum negatively affected the development of embryos in a dose-dependent manner. With lower doses (≤ 20 µg/ml), mouse embryos were not apparently damaged. With very high doses (≥ 40 µg/ml), embryo quality significantly decreased. This assessment of the effect of molybdenum on the preimplantation embryo is an initial survey of toxicological risk.</p> </abstract>