The mannan of Candida albicans lacking β-1, 2-linked oligomannosides increases the production of inflammatory cytokines by dendritic cells. (May 2013)

• Record Type: Journal Article
• Authors: Ueno, Keigo; Okawara, Akiko; Yamagoe, Satoshi; Naka, Takashi; Umeyama, Takashi; Utena-Abe, Yuki; Tarumoto, Norihito; Niimi, Masakazu; Ohno, Hideaki; Doe, Matsumi; Fujiwara, Nagatoshi; Kinjo, Yuki; Miyazaki, Yoshitsugu
• Is Part Of: Medical mycology. Volume 51:Number 4(2013)
• Page Start: 385
• Page End: 395
• Publication Date: 2013-05
• Subjects: Medical mycology -- Periodicals; Veterinary mycology -- Periodicals; Mycology -- Periodicals; Mycoses -- Periodicals; Pathogenic fungi -- Periodicals; 616.969005
• DOI: 10.3109/13693786.2012.733892
• Languages: English
• ISSNs: 1369-3786

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <p>Mannans are mannose polymers attached to cell wall proteins in all <italic>Candida</italic> species, including the pathogenic fungus <italic>Candida albicans.</italic> Mannans are sensed by pattern recognition receptors expressed on innate immune cells. However, the detailed structural patterns affecting immune sensing are not fully understood because mannans have a complex structure that includes α- and β-mannosyl linkages. In this study, we focused on the β-1, 2-mannosides of <italic>N</italic>-linked mannan in <italic>C. albicans</italic> because this moiety is not present in the non-pathogenic yeast <italic>Saccharomyces cerevisiae</italic>. To investigate the impact of β-1, 2-mannosides on immune sensing, we constructed a <italic>C. albicans</italic> ∆<italic>mnn4</italic>/∆<italic>bmt1</italic> double deletant. Thin-layer chromatography and nuclear magnetic resonance analyses revealed that the deletant lacked β-1, 2-mannosides in <italic>N</italic>-linked mannan. Mannans lacking the β-1, 2-mannosides induced the production of higher levels of inflammatory cytokines, including IL-6, IL-12p40 and TNF-α, in mice dendritic cells compared to wild-type mannan. Our data show that β-1, 2-mannosides in <italic>N</italic>-linked mannan reduce the production of inflammatory cytokines by dendritic cells.</p> </abstract>