Adenovirus-mediated bone morphogenetic protein-2 gene transfection of bone marrow mesenchymal stem cells combined with nano-hydroxyapatite to construct bone graft material in vitro. (April 2013)

• Record Type: Journal Article
• Authors: Li, W. C.; Wang, D. P.; Li, L. J.; Zhu, W. M.; Zeng, Y. J.
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 41:Number 2(2013:Apr.)
• Page Start: 103
• Page End: 108
• Publication Date: 2013-04
• Subjects: Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.3109/10731199.2012.715088
• Languages: English
• ISSNs: 2169-1401

Abstract

<abstract> <title>Abstract</title> <p>To study the adhesion, proliferation and expression of bone marrow mesenchymal stem cells (BMSCs) on nano-hydroxyapatite (Nano-HA) bone graft material after transfection of adenovirus-mediated human bone morphogenetic protein-2 expression vector (Ad-BMP-2). BMSCs were transfected using Ad-BMP-2. Immunohistochemistry and Western blot were used to detect BMP-2 expression in transfected cells. After transfection, BMP-2 protein was highly expressed in BMSCs; MTT test assay showed that the Nano-HA bone graft material could not inhibit <italic>in vitro</italic> proliferation of BMSCs. Ad-BMP-2-transfected BMSCs are well biocompatible with Nano-HA bone graft material, the transfected cells in material can secrete BMP-2 stably for a long time.</p> </abstract>