Prosaposin-derived peptide alleviates ischaemia-induced hearing loss. (May 2013)

• Record Type: Journal Article
• Authors: Terashita, Takehiro; Saito, Shouichiro; Nabeka, Hiroaki; Hato, Naohito; Wakisaka, Hiroyuki; Shimokawa, Tetsuya; Kobayashi, Naoto; Gyo, Kiyofumi; Matsuda, Seiji
• Is Part Of: Acta oto-laryngologica. Volume 133:Number 5(2013)
• Page Start: 462
• Page End: 468
• Publication Date: 2013-05
• Subjects: Otolaryngology -- Periodicals; Ear -- Diseases -- Periodicals; Throat -- Diseases -- Periodicals; Otolaryngology -- Electronic Resources; Otorhinolaryngologic Diseases; 617.8
• DOI: 10.3109/00016489.2012.750430
• Languages: English
• ISSNs: 0001-6489

Abstract

<abstract> <title>Abstract</title> <p> <italic>Conclusion:</italic> An 18-mer peptide derived from the neurotrophic region of prosaposin (PS-pep) prevents hearing loss and cochlear damage due to transient cochlear ischaemia by activating an anti-apoptotic pathway. PS-pep is a potent candidate molecule for alleviating ischaemia-induced hearing loss. <italic>Objective:</italic> PS-pep was investigated for its protective effects against ischaemia-induced hearing loss and cochlear damage. <italic>Methods:</italic> Ischaemia was induced in both cochleae in Mongolian gerbils by pulling the ligatures around both vertebral arteries in an anterior direction using 5 g weights for 15 min. PS-pep was synthesized artificially and administered subcutaneously four times after the induction of transient cochlear ischaemia. <italic>Results:</italic> An increase in the auditory brainstem response threshold was alleviated in animals treated with 2.0 mg/kg PS-pep. Histological examinations conducted on day 7 showed that the loss of inner hair cells (IHCs) was more prominent than that of outer hair cells. Higher doses of PS-pep significantly alleviated IHC loss. An increase in the anti-apoptotic factor bcl-2 was also noted in the IHCs treated with PS-pep.</p> </abstract>