Microarray Analysis of Altered Gene Expression and the Role of ATF3 in HK-2 Cells Treated with Hemin. (June 2013)

• Record Type: Journal Article
• Authors: Wang, Jingwen; Wang, Dewen; Li, Yang; Gao, Yabing; Wang, Shaoxia; Zuo, Hongyan; Xu, Xinping; Wang, Shuiming; Peng, Ruiyun
• Is Part Of: Renal failure. Volume 35:Number 5(2013)
• Page Start: 624
• Page End: 632
• Publication Date: 2013-06
• Subjects: Chronic renal failure -- Periodicals; Acute renal failure -- Periodicals; Uremia -- Periodicals; 616.614005
• DOI: 10.3109/0886022X.2013.780619
• Languages: English
• ISSNs: 0886-022X

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Objective</italic>: To identify gene expression changes and the role of activating transcription factor 3 (ATF3) in hemin toxicity in renal tubular epithelial cells, then elucidate molecular mechanisms of hemin toxicity on renal tubular epithelial cells.</p> <p> <italic>Methods</italic>: An oligo array comprising 35, 035 genes was used to compare differential gene expression in hemin-treated and non-treated HK-2 cells (human renal proximal tubular epithelial cells), and the role of ATF3 in hemin toxicity was assessed using siRNA technique.</p> <p> <italic>Results</italic>: A total of 128 mRNAs were at least twofold up-regulated and 101 mRNAs were at least twofold down-regulated after hemin treatment. Expression levels of ATF3, heat shock protein 70, c-fos, and c-jun were remarkably increased. Hemin also suppressed nuclear factor-kappa B inhibitor α, β-2 adrenergic receptor, and interleukin-6 mRNA amounts more than twofold. We further demonstrated the protective role of ATF3 in hemin cytotoxicity.</p> <p> <italic>Conclusions</italic>: The data suggest that hemin caused multiple changes of gene expression in HK-2 cells, and ATF3 protects against hemin cytotoxicity.</p> </abstract>