Silibinin attenuates oxidative metabolism and cytokine production by monocytes from preeclamptic women. (April 2013)

• Record Type: Journal Article
• Authors: Cristofalo, R.; Bannwart-Castro, C. F.; Magalhães, C. G.; Borges, V. T. M.; Peraçoli, J. C.; Witkin, S. S.; Peraçoli, M. T.
• Is Part Of: Free radical research. Volume 47:Number 4(2013:Apr.)
• Page Start: 268
• Page End: 275
• Publication Date: 2013-04
• Subjects: Free radicals (Chemistry) -- Periodicals; Antioxidants -- Periodicals; Vitamin C -- Periodicals; Vitamin E -- Periodicals; 541.224
• DOI: 10.3109/10715762.2013.765951
• Languages: English
• ISSNs: 1071-5762

Abstract

<abstract> <title>Abstract</title> <p>Silibinin is a polyphenolic plant flavonoid with anti-inflammatory properties. The present study investigated the effect of silibinin on oxidative metabolism and cytokine production - tumor necrosis factor-alpha (TNF-α), interleukin (IL)12, granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-6, IL-10, and transforming growth factor beta (TGF-β<sub>1</sub>) - by peripheral blood monocytes (PBM) from preeclamptic pregnant women. It is a case-controlled study involving women with preeclampsia (PE, n = 30) compared with normotensive pregnant (NT, n = 30) and with non-pregnant (NP, n = 30) women. Monocytes were obtained and cultured with or without silibinin (5 μM or 50 μM) for 18 h. Superoxide anion (O<sub>2</sub>−) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) release were determined by specific assays, and cytokine levels were determined by immunoenzymatic assays (ELISA). Monocytes from preeclamptic women cultured without stimulus released higher levels of O<sub>22</sub>, H<sub>2</sub>O<sub>2</sub> and TNF-α, and lower levels of IL-10 and TGF-β<sub>1</sub> than did monocytes from NT and NP women. Treatment in vitro with silibinin significantly inhibited spontaneous O<sub>2</sub>− and H<sub>2</sub>O<sub>2</sub> release and TNF-α production by monocytes from preeclamptic women. The main effect of silibinin was obtained at 50 μM concentration. Thus, silibinin exerts anti-oxidative and anti-inflammatory effects on monocytes from preeclamptic pregnant women by inhibiting the in vitro endogenous release of reactive oxygen species and TNF-α production.</p> </abstract>