Anti-inflammatory, analgesic, and antioxidant activities of Pisonia aculeata: Folk medicinal use to scientific approach. (April 2013)

• Record Type: Journal Article
• Authors: Sen, Saikat; Chakraborty, Raja; Rekha, B.; Revathi, D.; Ayyanna, S. Chinna; Hemalatha, G.; Kumar Reddy, G. Ashok; Hyndavi, S.; Ikhyatha Babu, P. Jeevan; Prakash, P. Ravi; Sridhar, C.
• Is Part Of: Pharmaceutical biology. Volume 51:Number 4(2013:Apr.)
• Page Start: 426
• Page End: 432
• Publication Date: 2013-04
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2012.738331
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Context</italic>: <italic>Pisonia aculeata</italic> leaves (Nyctagenaceae), a Folk medicinal plant used in the treatment of several inflammation, pain, and oxidative stress associated diseases.</p> <p> <italic>Objective</italic>: To evaluate anti-inflammatory, analgesic, and antioxidant potential of crude methanol extract of <italic>P. aculeata</italic> leaves (MEPA).</p> <p> <italic>Materials and methods</italic>: Analgesic and anti-inflammatory activities of MEPA (250 and 500 mg/kg) were evaluated using writhing, formalin, hot plate, tail flick, carrageenan-induced paw edema test, and membrane stabilizing activity. Free radical scavenging activity, total phenolic and flavonoid contents of MEPA were also determined using standard methods.</p> <p> <italic>Results</italic>: Oral administration of MEPA showed significant (p < 0.001) inhibition of paw edema, pronounced at 4 h and 5 h after carrageenan injection, and at 200 µg/mL exerts 77.67 and 38.51% protective effect against hypotonic solution and heat induced hemolysis, respectively. MEPA (250 and 500 mg/kg) produced 35.21 and 79.14% inhibition of acetic acid-induced writhing. Furthermore, MEPA (500 mg/kg) inhibited 49.19% early and 73.14% late phase of formalin-induced hypernociception. In contrast, a lower dose of MEPA did not prevent hot plate induced nociception, while in the tail immersion method, pronounced analgesic activity was observed between 1 and 4 h postdosing. The extract possesses significant <italic>in vitro</italic> antioxidant activity and a lipid peroxidation inhibition effect. Total phenolic and total flavonoid content in MEPA were 87.99 ± 0.87 mg GAE/g and 58.98 ± 0.01 mg QE/g, respectively.</p> <p> <italic>Discussion and conclusion</italic>: Our findings confirmed the analgesic, anti-inflammatory and antioxidant activities of <italic>Pisonia aculeata</italic> leaves. Contents of flavonoids and phenolic compounds in extract could be correlated with its observed biological activities.</p> </abstract>